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CTLA-4 His Tag Protein, Human

Cytotoxic T-lymphocyte-associated protein 4

价格 ￥1,050.00 （ 供应商现货 ： 3-5个工作日）
货号 UA010077
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • 分子别名

    Cytotoxic T-lymphocyte-associated protein 4

  • Accession

    P16410

  • 表达序列

    Ala37 - Phe162, with C-terminal 10*His

  • 表达宿主

    HEK293

  • 分子量

    20-27kDa (Reducing)

  • 纯度

    ＞95% by SDS-PAGE

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    His Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.
背景介绍
  • Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) is an inhibitory receptor belonging to the CD28 immunoglobulin subfamily, expressed primarily by T-cells. The family includes CD28, CTLA-4 and ICOS as well as other proteins including PD-1, BTLA and TIGIT.Its ligands, CD80 and CD86, are typically found on the surface of antigen-presenting cells and can either bind CD28 or CTLA-4, resulting in a costimulatory or a co-inhibitory response, respectively. Because of its dampening effect, CTLA-4 is a crucial regulator of T-cell homeostasis and self-tolerance. The mechanisms by which CTLA-4 exerts its inhibitory function can be categorized as either cell-intrinsic (affects the CTLA-4 expressing T-cell) or cell-extrinsic (affects secondary cells). CTLA-4 mainly acts in a cell-extrinsic manner via its competition with CD28, CTLA-4-mediated trans-endocytosis of CD80 and CD86, and its direct tolerogenic effects on the interacting cell.

  • 生物活性

    • Immobilized CTLA-4 His Tag, Human (Cat. No. UA010077) at 2μg/ml (100μl/well) can bind B7-1/CD80 Fc Chimera, Human (Cat. No. UA010031) with EC50 of 11.58ng/ml.
    • Anti-His antibody Immobilized on CM5 Chip captured CTLA-4 His Tag, Human (Cat. No. UA010077), can bind B7-1/CD80 Fc Chimera, Human (Cat. No. UA010031) with an affinity constant of 12.45nM as determined in SPR assay.
    • Anti-His antibody Immobilized on CM5 Chip captured CTLA-4 His Tag, Human (Cat. No. UA010077), can bind B7-2/CD86 Fc Chimera, Human (Cat. No. UA010187) with an affinity constant of 0.12 μM as determined in SPR assay.
    • Immobilized CTLA-4 His Tag, Human (Cat. No. UA010077) at 1.5μg/mL (100μL/well) can bind B7-2/CD86 Fc Chimera, Human (Cat. No. UA010187) with EC50 of 0.074-0.094μg/ml.

  • 电泳

    • 1μg（R: reducing conditions, N: non-reducing conditions).

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