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产品中心 药物靶点和Fc受体 药物靶点

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CTLA-4 Fc Chimera Protein, Human

价格 ￥1,050.00 （ 供应商现货 ： 3-5个工作日）
货号 UA010039
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 物种

    Human

  • Accession

    P16410

  • 表达序列

    Ala37-Phe162, with C-terminal Human IgG1 Fc

  • 表达宿主

    HEK293

  • 分子量

    49-54 kDa(Reducing)

  • 纯度

    ＞95%, by SDS-PAGE under reducing conditions

    ＞95%，as determined by HPLC

  • 内毒素含量

    <0.1EU/μg

  • 标记

    Unconjugated

  • 标签

    Human Fc Tag

  • 性状

    Lyophilized Powder

  • 缓冲体系

    PBS, pH7.4

  • 溶解方法

    Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation.

  • 储存条件

    · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.
    · 3 months, -20 to -80℃ under sterile conditions after reconstitution.
    · 1 week, 2 to 8℃ under sterile conditions after reconstitution.
    · Please avoid repeated freeze-thaw cycles.
背景介绍

  • Cytotoxic T-lymphocyte-associated protein 4 (CTLA-4) is an inhibitory receptor belonging to the CD28 immunoglobulin subfamily, expressed primarily by T-cells. The family includes CD28, CTLA-4 and ICOS as well as other proteins including PD-1, BTLA and TIGIT.Its ligands, CD80 and CD86, are typically found on the surface of antigen-presenting cells and can either bind CD28 or CTLA-4, resulting in a costimulatory or a co-inhibitory response, respectively. Because of its dampening effect, CTLA-4 is a crucial regulator of T-cell homeostasis and self-tolerance. The mechanisms by which CTLA-4 exerts its inhibitory function can be categorized as either cell-intrinsic (affects the CTLA-4 expressing T-cell) or cell-extrinsic (affects secondary cells). CTLA-4 mainly acts in a cell-extrinsic manner via its competition with CD28, CTLA-4-mediated trans-endocytosis of CD80 and CD86, and its direct tolerogenic effects on the interacting cell.

  • 生物活性

    • Anti-His antibody Immobilized on CM5 Chip captured B7-1/CD80 His Tag, Human (Cat. No. UA010085), can bind CTLA-4 Fc Chimera, Human (Cat. No. UA010039) with an affinity constant of 8.432nM as determined in SPR assay (Biacore T200).
    • Anti-His antibody Immobilized on CM5 Chip captured B7-2/CD86 His Tag, Human (Cat. No. UA010098), can bind CTLA-4 Fc Chimera, Human (Cat. No. UA010039) with an affinity constant of 49.58nM as determined in SPR assay (Biacore T200).
    • Immobilized B7-1/CD80 His Tag, Human (Cat. No. UA010085) at 5μg/mL (100 μL/well) can bind CTLA-4 Fc Chimera, Human (Cat. No. UA010039) with EC50 of 85.96ng/mL.
    • Immobilized B7-2/CD86 His Tag, Human (Cat. No. UA010098) at 5μg/mL (100μL/well) can bind CTLA-4 Fc Chimera, Human (Cat. No. UA010039) with EC50 of 33.50ng/mL.

  • 电泳

    • CTLA-4 Fc Chimera, Human, 2μg（R: reducing conditions, N: non-reducing conditions).

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