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产品中心 药物靶点和Fc受体 药物靶点

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CD47 His Tag Protein, Human

CD47,antigen identified by monoclonal 1D8,Antigenic surface determinant protein OA3,CD47 antigen (Rh-related antigen,integrin-associated signal transducer),CD47 antigen,CD47 glycoprotein,CD47 molecule,IAP,IAPintegrin associated protein,Integrin-associated protein,leukocyte surface antigen CD47,MER6,MER6integrin-associated signal transducer,OA3,Protein MER6,Rh-related antigen

价格 ￥1,300.00 （ 1-2周）
货号 UA010014
规格
数量
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  • Biotinylated CD47 Fc Chimera Protein, Human

    规格：25μg

    价格：￥2,700.00

    • 产品介绍

    产品介绍 评论(0)

    产品规格

    • 物种

      Human

    • 分子别名

      CD47 ;antigen identified by monoclonal 1D8, Antigenic surface determinant protein OA3, CD47 antigen (Rh-related antigen, integrin-associated signal transducer), CD47 antigen, CD47 glycoprotein, CD47 molecule, CD47, IAP, IAPintegrin associated protein, Integrin-associated protein, leukocyte surface antigen CD47, MER6, MER6integrin-associated signal transducer, OA3, Protein MER6, Rh-related antigen

    • Accession

      Q08722

    • 表达序列

      Gln19-Pro139, with C-terminal His Tag

    • 表达宿主

      HEK293

    • 分子量

      33-45 kDa(Reducing)

    • 纯度

      ＞95%, by SDS-PAGE under reducing conditions

    • 内毒素含量

      <0.2EU/μg

    • 标记

      Unconjugated

    • 标签

      His Tag

    • 性状

      Lyophilized Powder

    • 缓冲体系

      PBS pH7.2、5%海藻糖

    • 溶解方法

      Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation .

    • 储存条件

      · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

      · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

      · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

      · Please avoid repeated freeze-thaw cycles.

    背景介绍

    • 白细胞表面抗原CD47，也被称作整合素相关蛋白（Integrin-associated protein, IAP），是所有细胞表达的靶点中“marker of self”的典型代表。CD47是一个52 kDa糖蛋白，具有免疫球蛋白可变的N端结构域、5个跨膜结构域和一个短的C端胞内尾，胞内尾部有四种可变不同剪接异构体，从而形成4个亚型。CD47是靶向肿瘤免疫治疗中发挥主要作用的免疫细胞类群，癌细胞表达的CD47与NK细胞表达的SIRP家族蛋白SIRPα以及SIRPγ相互作用，通过这样的方式保护癌细胞避免被吞噬消除。CD47在多种实体瘤细胞及恶性血液瘤细胞上呈现高表达，而且其表达水平与疾病进展呈正相关。 本产品系由HEK293细胞株重组表达的人源CD47，添加5%海藻糖、分装冻干制成。

    • 生物活性

      • Immobilized CD47 His Tag, Human (Cat. No. UA010014) at 1μg/mL (100μL/well) can bind SIRP-α Fc Chimera, Human(Cat. No. UA010005) with EC50 of 4.51-7.55 ng/mL

    • 电泳

      • CD47 His tag, Human,1μg on SDS-PAGE under Non-reducing and reducing condition. The purity is greater than 95%.

    • ELISA

      • Immobilized CD47 His Tag, Human (Cat. No. UA010014) at 2.0μg/mL (100μL/well) can bind Anti-Human CD47 Monoclonal Antibody (Lembio) with EC50 of 7.67-8.58ng/mL.

      • Immobilized SIRP-α Fc Chimera Protein, Human (Cat. No. UA010005 ) at 2.0μg/mL (100μL/well) can bind CD47 His Tag Protein, Human (Cat. No. UA010014) with EC50 of 0.77-0.97ng/mL.

    • SPR

      • Anti-His antibody Immobilized on CM5 Chip captured CD47 His tag, Human (Cat. No. UA010014), can bind SIRP-α Fc Chimera, Human(Cat. No. UA010005) with an affinity constant of 3.151 nM as determined in SPR assay.

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