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产品中心 药物靶点和Fc受体 药物靶点

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PD-1 His Tag Protein, Human

Programmed cell death-1,CD279,Programmed death receptor 1

价格 ￥900.00 （ 供应商现货 ： 3-5个工作日）
货号 UA010004
规格
数量
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    • 产品介绍

    产品介绍 评论(0)

    产品规格

    • 物种

      Human

    • 分子别名

      Programmed cell death-1, CD279, Programmed death receptor 1

    • Accession

      Q15116-1

    • 表达序列

      Leu25-Gln167, with C-terminal His Tag

    • 表达宿主

      HEK293

    • 分子量

      28-36 kDa (Reducing)

    • 纯度

      ＞95%, by SDS-PAGE under reducing conditions.

    • 内毒素含量

      <0.1EU/μg

    • 标记

      Unconjugated

    • 标签

      His Tag

    • 性状

      Lyophilized Powder

    • 缓冲体系

      PBS, pH7.4, 5% trehalose

    • 溶解方法

      Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation .

    • 储存条件

      · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃.

      · 3 months, -20 to -80℃ under sterile conditions after reconstitution.

      · 1 week, 2 to 8℃ under sterile conditions after reconstitution.

      · Please avoid repeated freeze-thaw cycles.

    背景介绍

    • PD-1(programmed death 1)程序性死亡受体1，是一种重要的免疫抑制分子，为免疫球蛋白超家族，是一个268氨基酸残基的膜蛋白。以PD-1为靶点的免疫调节对抗肿瘤、抗感染、抗自身免疫性疾病及器官移植存活等均有重要的意义。其配体PD-L1也可作为靶点，PD-1和PD-L1结合启动T细胞的程序性死亡，使肿瘤细胞获得免疫逃逸。 本产品系由HEK293细胞株表达人源PD-1，C-6His重组蛋白，经多步纯化后过滤、添加5%海藻糖、分装冻干制成。

    • 生物活性

      • Immobilized PD-1 His Tag, Human (Cat. No. UA010004) at 1.5μg/mL (100μL/well) can bind PD-L1 Fc Chimera, Human (Cat. No. UA010003) with an EC50 of 0.141-0.174µg/mL.

      • Immobilized PD-1 His Tag, Human (Cat. No. UA010004) at 0.5μg/mL (100μL/well) can bind Nivolumab (starter-bio, Cat. No. S0B0566) with EC50 of 2.24-2.98 ng/mL.

      • Immobilized PD-1 His Tag, Human (Cat. No. UA010004) at 0.5μg/mL (100μL/well) can bind Pembrolizumab (starter-bio, Cat. No. S0B0233) with EC50 of 1.28-1.49 ng/mL.

    • 电泳

      • PD-1 His Tag, Human ,1μg on SDS-PAGE under reducing and Non-reducing condition. The purity is greater than 95%.

    • SPR

      • PD-1 His Tag, Human (Cat. No. UA010004) captured on CM5 chip via anti-His antibody, can bind PD-L1 Fc Chimera, Human (Cat. No. UA010003) with an affinity constant of 0.6644 μM as determined in a SPR assay (Biacore T200).

      • Loaded PD-L1 Fc Chimera, Human (Cat. No. UA010003) on Protein A Biosensor, can bind PD-1 His Tag, Human (Cat. No. UA010004) with an affinity constant of 1.6 μM as determined in BLI assay.

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