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宿主来源
Human突变
Mutation in exon1浓度
2 mg/ml性状
Liquid缓冲体系
Supplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 1% v/v β-Mercaptoethanol, 0.01% w/v bromophenol blue or phenol red.
储存条件
-20 °C for 1 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.产品详情
SMAD2-Knockdown HeLa Cell Lysate is a lysate derived from a polyclonal population of HeLa cells in which SMAD2 expression has been knocked down using CRISPR/Cas9 gene editing. The knockdown efficiency has been confirmed by Western blot, and the targeted mutation is located within exon 3 of the SMAD2 gene. A control lysate from non-targeting HeLa cells is included for comparison.
Key Notes:
Boil for 3~5 minutes prior to use.
Prepared from cells lysed in RIPA buffer (supplemented with protease and phosphatase inhibitors).
Positive Control: SMAD2-knockdown HeLa cell lysate supplied in SDS sample buffer with bromophenol blue.
Negative Control: Control HeLa cell lysate supplied in SDS sample buffer with phenol red.应用
WB
SMAD2, also known as MADR2 or JV18-1, is a 58 kDa signaling protein widely expressed in various tissues, including epithelial and mesenchymal cells. It functions as a central intracellular mediator of the transforming growth factor-beta (TGF-β) receptor signaling pathway. Upon TGF-β stimulation, SMAD2 undergoes phosphorylation at serine residues, converting it into its active phosphorylated form. This activated SMAD2 then forms a heteromeric complex with SMAD4, which translocates into the nucleus. Within the nucleus, the complex binds to specific DNA sequences and regulates the transcription of target genes involved in critical biological processes such as cell proliferation, differentiation, apoptosis, and extracellular matrix production. Furthermore, SMAD2 is a key component of the activin receptor signaling pathway and cooperates with other proteins like SMAD3 to precisely modulate cellular responses to external stimuli, thereby maintaining cellular homeostasis. Dysregulation of SMAD2 signaling is closely associated with various pathological conditions, including fibrosis and cancer, where its excessive activation promotes fibrotic tissue deposition and tumor progression. Therefore, a deeper understanding of the regulatory mechanisms of SMAD2 is essential for developing therapeutic strategies targeting these diseases.
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