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宿主来源
Human突变
Mutation in exon3浓度
2 mg/ml性状
Liquid缓冲体系
Supplied in SDS Sample Buffer: 50 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 1% v/v β-Mercaptoethanol, 0.01% w/v bromophenol blue or phenol red.
储存条件
-20 °C for 1 months under sterile conditions;
-80 °C for 24 months under sterile conditions.
Please avoid repeated freeze-thaw cycles.产品详情
IKK gamma-Knockdown HeLa Cell Lysate is a lysate derived from a polyclonal population of HeLa cells in which IKK-gamma expression has been knocked down using CRISPR/Cas9 gene editing. The knockdown efficiency has been confirmed by Western blot, and the targeted mutation is located within exon 3 of the IKK-gamma gene. A control lysate from non-targeting HeLa cells is included for comparison.
Key Notes:
Boil for 3~5 minutes prior to use.
Prepared from cells lysed in RIPA buffer (supplemented with protease and phosphatase inhibitors).
Positive Control: IKK-gamma-knockdown HeLa cell lysate supplied in SDS sample buffer with bromophenol blue.
Negative Control: Control HeLa cell lysate supplied in SDS sample buffer with containing phenol red.应用
WB
IKK-gamma, also known as NEMO or IKBKG, is a key regulatory protein with a molecular weight of approximately 46-50 kDa. Its structure is characterized by coiled-coil domains, enabling it to form a tetramer that functions as a molecular scaffold. The core biological function of Ikk-gamma is to serve as an essential regulatory subunit of the IKK kinase complex; it is responsible for receiving upstream signals and facilitating the assembly and activation of the complex. Within signaling pathways, Ikk-gamma orchestrates the activation of the canonical NF-κB pathway: upon cellular stimulation by agents such as TNFα or LPS, Ikk-gamma recognizes ubiquitination signals, leading to IKK complex activation. This, in turn, triggers the phosphorylation and subsequent degradation of the inhibitory protein IκB, ultimately releasing the NF-κB transcription factor for nuclear translocation. This process initiates the expression of genes involved in immunity, inflammation, and cell survival. Given its pivotal role, mutations in its gene can lead to immune disorders such as NEMO deficiency syndrome, and its dysregulation is intimately linked to cancer and chronic inflammatory diseases.
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