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产品中心 Multiplex 单因子自由搭配 Star-plex(Luminex平台)

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Monkey MCP-1/CCL2 Single Plex Assay (适用于Luminex)

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货号 S0X3206
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    MCP-1/CCL2

  • 反应种属 ?

    Monkey

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: ＜10%；
      Inter-assay: ＜15%

    • Sample type

      Serum; Plasma; Cell culture supernatant

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      ＜5 pg/mL

    • Range

      5 pg/mL-10000pg/mL

    • Recovery

      Plasma: 80%-120%
      Serum: 80%-120%
      Cell culture supernatant: 80%-120%

    • Assay time

      70 minutes

    • Species reactivity

      Monkey

  • Luminex

    • Standard curve
      Example of MCP-1/CCL2 standard curve in Assay Diluent .

    • Quantifiction of Monkey PBMC
      Stimulated sample: Monkey peripheral blood mononuclear cells (PBMCs) were stimulated with 10 ng/mL PMA for 24h, and the average concentration of MCP-1/CCL2 in the supernatant was measured to be 333.61 pg/mL.
      Unstimulated sample: Monkey PBMCs were cultured for 24h, and the average concentration of MCP-1/CCL2 in the supernatant was measured to be 52.22 pg/mL.

    • Linearity
      The high-concentration monkey MCP-1/CCL2 protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively. Then, the concentration of monkey MCP-1/CCL2 was determined and interpolated based on the target standard curve, and the sample dilution was corrected.

    • Recovery
      Within the detection range, the combined cytokine proteins were added to serum,plasma,cell supernatant at high、low concentrations.The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins. The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.

    • Protocol Diagram

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