产品中心 多因子检测试剂盒 Star-Plex Multiplex（适用Luminex平台)
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抗原名称
TNF-α反应种属 ?
Mouse储存条件
12 months from date of receipt / reconstitution, 2 to 8°C as supplied.
Precision
Intra-assay: ＜10%；
Inter-assay: ＜20%Sample type
Serum; Plasma; Cell culture supernatantAssay type
Sandwich (quantitative)Sensitivity
＜5 pg/mLRange
4.5pg/mL-200000pg/mLRecovery
Plasma: 80%-120%
Serum: 80%-120%
Cell culture supernatant: 80%-120%Assay time
70 minutesSpecies reactivity
Mouse
Luminex
Standard curve
Example of TNF-α standard curve in Assay Diluent .Quantifiction of Mouse spleen cells
Stimulated sample: Mouse spleen cells were stimulated with 4 ug/mL ConA for 24h, and the average concentration of TNF-α in the supernatant was measured to be 511.51 pg/mL.
Unstimulated sample: Mouse spleen cells were cultured for 24h, and the average concentration of TNF-α in the supernatant was measured to be 2.12 pg/mL.Linearity
The high-concentration Mouse TNF-α protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively. Then, the concentration of Mouse TNF-α was determined and interpolated based on the target standard curve, and the sample dilution was corrected.Recovery
Within the detection range, the cytokine proteins were added to serum,plasma,cell supernatant at high、low concentrations.The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins. The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.Protocol Diagram
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