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产品中心 Multiplex 单因子自由搭配 Star-plex(Luminex平台)

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Human IL-12p70 Single Plex Assay (适用于Luminex)

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货号 S0X3123
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产品介绍

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产品规格

  • 抗原名称

    IL-12p70

  • 反应种属 ?

    Human

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: ＜10%；
      Inter-assay: ＜15%

    • Sample type

      Serum; Plasma; Cell culture supernatant

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      ＜0.1 pg/mL

    • Range

      0.5pg/mL-5000pg/mL

    • Recovery

      Plasma: 80%-120%
      Serum: 80%-120%
      Cell culture supernatant: 80%-120%

    • Assay time

      70 minutes

    • Species reactivity

      Human

  • Luminex

    • Standard curve
      Example of IL-12p70 standard curve in Assay Diluent .

    • Quantifiction of human PBMC
      Stimulated sample: Human peripheral blood mononuclear cells (PBMCs) were stimulated with 10 ng/mL PMA for 24h, and the average concentration of IL-12p70 in the supernatant was measured to be 36.22 pg/mL.
      Unstimulated sample: Human PBMCs were cultured for 24h, and the average concentration of IL-12p70 in the supernatant was measured to be 1.47 pg/mL.

    • Linearity
      The high-concentration human IL-12p70 protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively. Then, the concentration of human IL-12p70 was determined and interpolated based on the target standard curve, and the sample dilution was corrected.

    • Recovery
      Within the detection range, the combined cytokine proteins were added to serum,plasma,cell supernatant at high、low concentrations.The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins. The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.

    • Protocol Diagram

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