Human TNF-α Single Plex Assay (适用于Luminex)

Luminex

• Standard curve
  Example of TNF-α standard curve in Assay Diluent .

• Quantifiction of human PBMC
  Stimulated sample: Human peripheral blood mononuclear cells (PBMCs) were stimulated with 10 ng/mL PMA for 24h, and the average concentration of TNF-α in the supernatant was measured to be 36.22 pg/mL.
  Unstimulated sample: Human PBMCs were cultured for 24h, and the average concentration of TNF-α in the supernatant was measured to be 1.47 pg/mL.

• Linearity
  The high-concentration human TNF-α protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively. Then, the concentration of human TNF-α was determined and interpolated based on the target standard curve, and the sample dilution was corrected.

• Recovery
  Within the detection range, the combined cytokine proteins were added to serum,plasma,cell supernatant at high、low concentrations.The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins. The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.

• Protocol Diagram