Human Th1/Th2/Inflammation 10-Plex Assay (适用于Luminex)

Luminex

• Linearity
  After serial dilution of the high-concentration combined proteins of IL-1β，IL-2, IL-4, IL-6, IL-8,IL-10, IL-12p70，IL-13,TNF-α,and IFN-γ using plasma, serum, and cell supernatant matrices respectively, the linear slopes between the actual detected values and the theoretical values are as shown in the table.

• Standard Curve
  Example of IL-1β，IL-2, IL-4, IL-6, IL-8,IL-10, IL-12p70，IL-13,TNF-α,and IFN-γ standard curve in Assay Diluent.

• Quantifiction of Human PBMC
  PBMC Unsimulated Supernatant: The cells of PBMCs were cultured for24 hours, the cell culture supernatant was collected and analyzed quantitatively using S0X1004.
  PBMC simulated Supernatant: The cells of PBMCs were stimulated with 10 μg/mL PMA. After 24 hours, the cell culture supernatant was collected and analyzed quantitatively using S0X1004.

• Intra-assay precision
  Ten replicates of each of three different levels of IL-1β，IL-2, IL-4, IL-6, IL-8,IL-10, IL-12p70，IL-13,TNF-α,and IFN-γ were tested.
  
  

• Inter-assay precision
  Ten repeated tests were conducted on three different levels of IL-1β，IL-2, IL-4, IL-6, IL-8,IL-10, IL-12p70，IL-13,TNF-α,and IFN-γ using different batches of reagents.

• Specificity
  The antibodies used in the kit have been screened for their specific reactivity with particular cytokines. After analyzing samples containing only a single recombinant cytokine protein, it was found that when using this detection method, there was no cross-reaction or background detection of cytokines in other capture magnetic bead groups.

• Theoretical limit of detection
  Repeat the detection of the blank sample 20 times using the same batch of reagents, and calculate the theoretical limit of detection.

• Protocol Diagram

• Recovery

  Within the detection range, the combined cytokine proteins were added to serum,plasma,cell supernatant at high concentrations.The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins. Theresults were compared with those of samples that had the same concentration of cytokines added in the assay buffer.