Human Cytokine Th1/Th2 Assay (适用于Luminex)

Luminex

• Linearity
  After serial dilution of the high-concentration combined proteins of IL-2, IL-4, IL-5,IL-10,IL-12p70,IL-13,TNF-α, IFN-γ and GM-CSF using plasma, serum, and cell supernatant matrices respectively, the linear slopes between the actual detected values and the theoretical values are as shown in the table.

• Standard Curve
  Example of IL-2, IL-4, IL-5,IL-10,IL-12p70,IL-13,TNF-α, IFN-γ and GM-CSF standard curve in Assay Diluent.

• Performance Characteristics

• Precision
  Ten replicates of each of three different levels of IL-2, IL-4, IL-5,IL-10,IL-12p70,IL-13,TNF-α, IFN-γand GM-CSF were tested.

• Specificity
  The antibodies used in the kit have been screened for their specific reactivity with particular cytokines. After analyzing samples containing only a single recombinant cytokine protein, it was found that when using this detection method, there was no cross-reaction or background detection of cytokines in other capture magnetic bead groups.

• Recovery
  Within the detection range, the combined cytokine proteins were added to serum,plasma,cell supernatant at high concentrations.The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins. Theresults were compared with those of samples that had the same concentration of cytokines added in the assay buffer.