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产品中心 多因子检测试剂盒 Star-Plex Multiplex（适用Luminex平台)

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Human Cytokine 10-Plex Assay (适用于Luminex)

价格 ￥24,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0X1001
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-1β、IL-2、IL-4、IL-5、IL-6、IL-8、IL-10、IL-13、TNF-α、IFN-γ

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Human

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8℃ as supplied.

试剂盒参数

    • Precision

      批内变异系数 (Intra-assay): 2.67%-7.76%；
      批间变异系数 (Inter-assay): 8.3%-13.76%

    • Sample type

      Cell culture supernatant, Serum, Plasma

    • Assay type

      Sandwich (Qualitative)

    • Sensitivity

      <1.0pg/ml

    • Range

      0.5-10000 pg/mL

    • Recovery

      70%-130%

    • Assay time

      70 minutes

    • Species reactivity

      Human

    • Plate

      Non-detachable plate

  • Luminex

    • Linearity
      After serial dilution of the high-concentration combined proteins of IL-1β，IL-2, IL-4, IL-5, IL-6, IL-8,IL-10, IL-13,TNF-α,and IFN-γusing plasma, serum, and cell supernatant matrices respectively, the linear slopes between the actual detected values and the theoretical values are as shown in the table.

    • Standard Curve
      Example of IL-1β, IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,IL-13,TNF-α,IFN-γstandard curve in Assay Diluent.

    • Performance Characteristics

    • Precision
      Ten replicates of each of three different levels of IL-1β, IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,IL-13,TNF-α,IFN-γwere tested.

    • Specificity
      The antibodies used in the kit have been screened for their specific reactivity with particular cytokines. After analyzing samples containing only a single recombinant cytokine protein, it was found that when using this detection method, there was no cross-reaction or background detection of cytokines in other capture magnetic bead groups.

    • Recovery
      Within the detection range, the combined cytokine proteins were added to serum,plasma,cell supernatant at high concentrations.The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins. Theresults were compared with those of samples that had the same concentration of cytokines added in the assay buffer.

    • Distribution Diagram（Luminex）
      Luminex Distribution diagram of Beads on the instrument operation software interface.

    • Distribution Diagram(CBA)
      Distribution diagram of Beads on the interface of flow cytometry operating software.

    • Distribution Diagram（CBA）
      Grouping diagram of Beads on the interface of flow cytometry operating software.

    • Distribution Diagram（CBA）
      Grouping diagram of Beads on the interface of flow cytometry operating software.

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