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产品中心 Multiplex 单因子自由搭配 FCM(流式平台)

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Monkey GM-CSF Single Plex Kit (Flow Cytometry Bead Assay)

价格 ￥3,000.00 （ 1-2周）
货号 S0Q3215
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    GM-CSF

  • 反应种属 ?

    Monkey

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 2.14%;
      Inter-assay: 6.01%

    • Sample type

      Serum; EDTA Plasma; Heparin Plasma; Citrate plasma; Cell culture supernatant

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      4.907 pg/mL

    • Range

      3.81 - 8000 pg/mL

    • Recovery

      70.94% - 96.36%

    • Assay time

      70 minutes

    • Species reactivity

      Monkey

  • CBA

    • Linearity
      The high-concentration monkey GM-CSF protein was serially diluted using serum, heparin plasma, EDTA plasma, citrate plasma and cell culture supernatant matrices respectively.
      The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
      Then, the concentration of monkey GM-CSF was determined and interpolated based on the target standard curve, and the sample dilution was corrected.

    • Standard curve
      Example of GM-CSF standard curve in Assay Diluent .

    • Recovery
      Within the detection range, the monkey GM-CSF protein was added to different matrices at high, medium, low concentrations.
      The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
      The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.

    • Quantitative analysis of monkey PBMC samples
      Stimulated sample: Monkey peripheral blood mononuclear cells (PBMCs) were stimulated with 10 ng/mL PMA and 1μg/mL Iono for 24h, and the average concentration of GM-CSF in the supernatant was measured to be 475.06 pg/mL.
      Unstimulated sample: Monkey PBMCs were cultured for 24h, and the average concentration of GM-CSF in the supernatant was measured to be 2.18 pg/mL.

    • Linearity
      The high-concentration monkey GM-CSF protein was serially diluted using serum, heparin plasma, EDTA plasma, citrate plasma and cell culture supernatant matrices respectively.
      The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
      Then, the concentration of monkey GM-CSF was determined and interpolated based on the target standard curve, and the sample dilution was corrected.

    • Protocol Diagram

    • Standard curve
      Example of GM-CSF standard curve in Assay Diluent .

    • Recovery
      Within the detection range, the monkey GM-CSF protein was added to different matrices at high, medium, low concentrations.
      The matrices used in these experiments were diluted 2-fold with the assay buffer before the addition of the cytokine proteins.
      The results were compared with those of samples that had the same concentration of cytokines added in the assay buffer.

    • Quantitative analysis of monkey PBMC samples
      Stimulated sample: Monkey peripheral blood mononuclear cells (PBMCs) were stimulated with 10 ng/mL PMA and 1μg/mL Iono for 24h, and the average concentration of GM-CSF in the supernatant was measured to be 475.06 pg/mL.
      Unstimulated sample: Monkey PBMCs were cultured for 24h, and the average concentration of GM-CSF in the supernatant was measured to be 2.18 pg/mL.

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