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产品中心 Multiplex 单因子自由搭配 FCM(流式平台)

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Monkey MCP-1/CCL2 Single Plex Kit (Flow Cytometry Bead Assay)

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货号 S0Q3206
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    MCP-1/CCL2

  • 反应种属 ?

    Monkey

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 1.44%；
      Inter-assay: 1.72%

    • Sample type

      Serum; Plasma; Cell culture supernatant

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.375 pg/mL

    • Range

      6.86 - 10000 pg/mL

    • Recovery

      Plasma: 115.63%
      Serum: 96.59%
      Cell culture supernatant: 128.32%

    • Assay time

      70 minutes

    • Species reactivity

      Monkey

  • CBA

    • Standard curve
      Example of MCP-1/CCL2 standard curve in Assay Diluent .

    • Quantifiction of Monkey PBMC
      Stimulated sample: Monkey peripheral blood mononuclear cells (PBMCs) were stimulated with 10 ng/mL PMA for 24h, and the average concentration of MCP-1/CCL2 in the supernatant was measured to be 333.61 pg/mL.
      Unstimulated sample: Monkey PBMCs were cultured for 24h, and the average concentration of MCP-1/CCL2 in the supernatant was measured to be 52.22 pg/mL.

    • Linearity
      The high-concentration monkey MCP-1/CCL2 protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively. Then, the concentration of monkey MCP-1/CCL2 was determined and interpolated based on the target standard curve, and the sample dilution was corrected.

    • Protocol Diagram

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