热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 Multiplex 单因子自由搭配 FCM(流式平台)

所有图片（1/4）

Human IL-4 Single Plex Kit (Flow Cytometry Bead Assay)

价格 ￥3,000.00 （ 1-2周）
货号 S0Q3047
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-4

  • 反应种属 ?

    Human

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3%；
      Inter-assay: 5%

    • Sample type

      Serum; Plasma; Cell culture supernatant

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.1 pg/mL

    • Range

      0.43 pg/mL-3500 pg/mL

    • Recovery

      Plasma: 110%
      Serum: 106%
      Cell culture supernatant: 122%

    • Assay time

      70 minutes

    • Species reactivity

      Human

  • CBA

    • Standard curve
      Example of IL-4 standard curve in Assay Diluent .

    • Quantifiction of human PBMC
      Stimulated sample: Human peripheral blood mononuclear cells (PBMCs) were stimulated with 10 ng/mL PMA for 24h, and the average concentration of IL-4 in the supernatant was measured to be 36.22 pg/mL.
      Unstimulated sample: Human PBMCs were cultured for 24h, and the average concentration of IL-4 in the supernatant was measured to be 1.47 pg/mL.

    • Linearity
      The high-concentration human IL-4 protein was serially diluted using plasma, serum and cell culture supernatant matrices respectively. Then, the concentration of human IL-4 was determined and interpolated based on the target standard curve, and the sample dilution was corrected.

    • Protocol Diagram

评论(0)