StepSlim Mouse IL-5 (HRP) ELISpot Kit

Elispot

• Linearity
  Three different mouse spleen cells concentrations, 8 replicates, 1 batch. Data presented the mean spot count, range, and coefficient of variation corresponding to the three cell concentrations.

• Precision
  Stimulate 4×10⁵ cells/well of mouse spleen cells with Con A for 20 hours at 37 °C in a 5% CO₂ incubator. Repeat across 8 wells in 3 batches to evaluate inter-batch and intra-batch precision. Data presented include mean spot count, range, and coefficient of variation.

• Diagram of spots
  Stimulate 4×10⁵ cells/well of mouse spleen cells with Con A. After 20 hours of stimulation at 37 °C in a 5% CO₂ incubator, incubate with HRP-labeled detection antibody at room temperature for 2 hours. Add substrate for color development, followed by image analysis and spot counting.