StepSlim Mouse Total IgG (HRP) ELISpot Kit

Elispot

• Linearity
  Mouse spleen cells were pre-incubated for 24 or 48 h in the presence of R848 and recombinant IL-2.
  Four different cryopreservation mouse spleen cells concentrations, 8 replicates, 1 batch. Data presented the mean spot count, range, and coefficient of variation corresponding to the four cell concentrations.

• Precision
  Mouse spleen cells were pre-incubated for 24 h in the presence of R848 and recombinant IL-2.
  Following pre-stimulation, the cells were washed and seeded at a density of 5 × 10⁴ cells per well following pre-stimulation, and incubated for 24 hours in a 37°C, 5% CO₂ incubator. Repeat across 8 wells in 3 batches to evaluate inter-batch and intra-batch precision. Data presented include mean spot count, range, and coefficient of variation.

• Diagram of spots
  After pre-stimulation of mouse spleen cells at 37°C with 5% CO₂ for 24 hours, the cells were incubated with HRP-labelled detection antibodies for 2 hours at room temperature. Then, a colour development substrate was added, followed by image analysis and spot counting.

• Protocol Diagram

• Competitor Comparison