StepSlim Human IL-5 (HRP) Plus ELISpot Kit

Elispot

• Linearity
  Three different cryopreservation PBMCs concentrations, 3 replicates, 1 batch. Data presented the mean spot count, range, and coefficient of variation corresponding to the three cell concentrations.

• Precision
  Stimulate 2×10⁵ cells/well of cryopreservation PBMCs with PMA and Ionomycin for 22 hours at 37°C in a 5% CO₂ incubator. Repeat across 8 wells in 3 batches to evaluate inter-batch and intra-batch precision. Data presented include mean spot count, range, and coefficient of variation.

• Diagram of spots
  Stimulate 2×10⁵ cells/well of cryopreserved PBMCs with PMA and Ionomycin. After 22 hours of stimulation at 37°C in a 5% CO₂ incubator, incubate with HRP-labeled detection antibody at room temperature for 2 hours. Add substrate for color development, followed by image analysis and spot counting.

• Protocol Diagram