StepSlim Human IFN-γ（HRP）plus ELISpot Kit

Elispot

• Precision
  Stimulate 1×10⁵ cells/well of cryopreservation PBMCs with PHA in a 37°C, 5% CO₂ incubator for 20 hours. Repeat across 6 wells in 3 batches. This evaluates inter-batch and intra-batch precision. Data presented includes mean spot count, range, and coefficient of variation.
  

• Linearity

  Five different cryopreservation PBMCs concentrations, 2 replicates, 1 batch. Data presented the mean spot count, range, and coefficient of variation corresponding to the five cell concentrations.
  

• Diagram of spots

  Stimulate 1×10⁵ cells/well of cryopreserved PBMCs with PHA. After 20 hours of stimulation at 37°C in a 5% CO₂ incubator, incubate with HRP-labeled detection antibody at room temperature for 2 hours. Add substrate for color development, followed by image analysis and spot counting.
  

• Protocol Diagram