产品中心 ELISPOT试剂盒 StepSlim ELISpot
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抗原名称
IL-2抗体类型
Recombinant mAb反应种属 ?
Mouse纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8℃ as supplied.
Precision
Intra-assay: 3.98%；
Inter-assay: 5.47%Sample type
Spleen cellsAssay type
Sandwich (Qualitative)Assay time
18-24 hoursSpecies reactivity
MousePlate
Strip
Mouse IL-2
Elispot
Linearity
Five different mouse spleen cells concentrations, 8 replicates, 1 batch. Data presented the mean spot count, range, and coefficient of variation corresponding to the five cell concentrations.Precision
Stimulate 2×10⁵ cells/well of mouse spleen cells with Con A for 22 hours at 37°C in a 5% CO₂ incubator. Repeat across 8 wells in 3 batches to evaluate inter-batch and intra-batch precision. Data presented include mean spot count, range, and coefficient of variation.Diagram of spots
Stimulate 2×10⁵ cells/well of mouse spleen cells with Con A. After 22 hours of stimulation at 37°C in a 5% CO₂ incubator, incubate with ALP-labeled detection antibody at room temperature for 2 hours. Add substrate for color development, followed by image analysis and spot counting.Protocol Diagram
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