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反应种属 ?
Ms阳性样本
Mouse splenocytes性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
| 货号 | 产品名称 | 推荐用量 | 规格 | 数量 |
|---|---|---|---|---|
| S0B1783-P | FITC Armenian hamster Anti-Mouse CD3 Antibody (S-R491) | 5μl/T | 50T | 1 |
| S0B8094-P | PE Rat Anti-Mouse CD49b Antibody (DX5) | 5μl/T | 50T | 1 |
| S0B8098-P | PE-Cy7 Mouse Anti-Mouse NK1.1 Antibody (PK136) | 5μl/T | 50T | 1 |
| S0B5500-P | APC Rat Anti-Mouse CD335 Antibody (S-R613) | 5μl/T | 50T | 1 |
This four‑color panel is designed for detecting natural killer (NK) cells in mouse tissue cells. CD3 was included as a T‑cell marker to exclude T lymphocytes, allowing the identification of NK cells within the CD3‑negative population. CD49b and NK1.1 are markers often used to detect NK cells; it is important to note that NK1.1 is also expressed on NKT cells, but since the analysis is performed on the CD3‑negative gate, NKT cells are excluded. Additionally, CD335 (NKp46) is included as another NK‑cell marker, which can be used together with NK1.1 to further confirm the NK cell identity. The panel is compatible with most two‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
流式分析
Schematic diagram of Mouse NK Cell Panel Kit
Mouse splenocytes were stained using the Mouse NK Cell Panel consisting of anti-mouse CD3 FITC, anti-mouse CD49b PE, anti-mouse NK1.1 PE-Cy7 and anti-mouse CD335 APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 777 (S0B88805) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs SSC-A and CD49b PE-A vs NK1.1 PE-Cy7-A dot plot show the NK cells with the CD3- gate applied.
4. Create a CD335 APC-A vs NK1.1 PE-Cy7-A dot plot show the NK cells with the CD3- gate applied.
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