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反应种属 ?
Ms阳性样本
Mouse bone marrow, Mouse splenocytes性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
| 货号 | 产品名称 | 推荐用量 | 规格 | 数量 |
|---|---|---|---|---|
| S0B5160-P | PerCP-Cy5.5 Rat Anti-Mouse/Human CD11b Antibody (M1/70) | 5μl/T | 50T | 1 |
| S0B5997-P | Pacific Blue Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Antibody (RB6-8C5) | 5μl/T | 50T | 1 |
| S0B5110-P | FITC Rat Anti-Mouse Ly-6C Antibody (HK1.4) | 5μl/T | 50T | 1 |
| S0B5168-P | APC Rat Anti-Mouse Ly6G Antibody (1A8) | 5μl/T | 50T | 1 |
| S0B5842-P | PE Rat Anti-Mouse CD115 Antibody (AFS98) | 5μl/T | 50T | 1 |
This five‑color panel is designed for detecting myeloid‑derived suppressor cell (MDSC) subsets in mouse tissue cells. CD11b was included as a general myeloid cell marker, and Ly‑6G/Ly‑6C (Gr‑1) was included to identify the total myeloid population . Ly6C and Ly6G were then used to distinguish monocytic MDSCs (M‑MDSC) and granulocytic MDSCs (G‑MDSC). Finally, CD115 (the M‑CSF receptor) was included to further characterize myeloid cells, as its expression can provide additional information on monocyte‑related populations or MDSC differentiation stages. The panel is compatible with most two‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
流式分析
Schematic diagram of Mouse MDSCs Panel Kit (CD11b/GR-1/LY6C/LY6G/CD115)
Mouse bone marrow were stained using the Mouse MDSCs Panel Kit consisting of anti-mouse CD11b PerCP-Cy5.5, anti-mouse Ly-6G/Ly-6C (Gr-1) Pacific Blue, anti-mouse Ly6C FITC, anti-mouse Ly6G APC and anti-mouse CD115 PE conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD11b PerCP-Cy5.5-A vs Ly-6G/Ly-6C (Gr-1) Pacific Blue-A dot plot show the myeloid cells with the live cells gate applied.
4. Create a Ly6G APC-A vs Ly6C FITC-A dot plot with the myeloid cells gate applied. Create gates to encompass Ly6G-Ly6Chi cells (M-MDSC), Ly6G+Ly6Gmid cells (G-MDSC)
5. Create a CD115 PE-A vs SSC-A dot plot with the CD11b+ gate applied.Mouse splenocytes were stained using the Mouse MDSCs Panel Kit consisting of anti-mouse CD11b PerCP-Cy5.5, anti-mouse Ly-6G/Ly-6C (Gr-1) Pacific Blue, anti-mouse Ly6C FITC, anti-mouse Ly6G APC and anti-mouse CD115 PE conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD11b PerCP-Cy5.5-A vs Ly-6G/Ly-6C (Gr-1) Pacific Blue-A dot plot show the myeloid cells with the live cells gate applied.
4. Create a Ly6G APC-A vs Ly6C FITC-A dot plot with the myeloid cells gate applied. Create gates to encompass Ly6G-Ly6Chi cells (M-MDSC), Ly6G+Ly6Gmid cells (G-MDSC)
5. Create a CD115 PE-A vs SSC-A dot plot with the CD11b+ gate applied.
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