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反应种属 ?
Hu阳性样本
Human peripheral blood cells性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
| 货号 | 产品名称 | 推荐用量 | 规格 | 数量 |
|---|---|---|---|---|
| S0B1636-P | Alexa Fluor® 488 Mouse Anti-Human CD3 Antibody (UCHT1) | 5μl/T | 50T | 1 |
| S0B1530-P | PE Mouse Anti-Human CD4 Antibody (S-574-16) | 5μl/T | 50T | 1 |
| S0B1551-P | Alexa Fluor® 647 Mouse Anti-Human CD8α Antibody (S-1036-34) | 5μl/T | 50T | 1 |
| S0B1583-P | APC-Cy7 Mouse Anti-Human CD45 Antibody (S-839-3) | 5μl/T | 50T | 1 |
This four‑color panel is designed for detecting T‑cell subpopulations in human peripheral blood or other biological samples. CD45 serves as a pan-leukocyte marker. CD3 was included as a general T‑cell marker, and CD4 and CD8 were included to identify helper T‑cell and cytotoxic T‑cell subpopulations. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
流式分析
Schematic diagram of Human T Cell Panel Kit
Human peripheral blood cells were stained using the Human T Cell Panel consisting of anti-human CD45 APC-Cy7, anti-human CD3 Alexa Fluor® 488, anti-human CD8 Alexa Fluor® 488 and anti-human CD4 PE conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the human peripheral blood cells excluding cellular debris.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the human peripheral blood cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 Alexa Fluor® 488-A vs SSC-A dot plot show the T cells and apply the CD45+ gate.
4. Create a CD8 Alexa Fluor® 647-A vs CD4 PE-A dot plot and apply the CD3+ gate to identify the T cells subpopulations.
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