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反应种属 ?
Hu阳性样本
Human peripheral blood cells性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
| 货号 | 产品名称 | 推荐用量 | 规格 | 数量 |
|---|---|---|---|---|
| S0B0155-P | Alexa Fluor® 647 Mouse Anti-Human CD3 Antibody (OKT3) | 5μl/T | 50T | 1 |
| S0B5931-P | PE Mouse Anti-Human CD56 Antibody (S-3499) | 5μl/T | 50T | 1 |
| S0B1632-P | Alexa Fluor® 488 Mouse Anti-Human CD16 Antibody (3G8) | 5μl/T | 50T | 1 |
This three‑color panel is designed for detecting natural killer (NK) cells in human peripheral blood or other biological samples. CD3 was included as a T‑cell marker to exclude T lymphocytes. CD56 is a marker often used to detect NK cells, and CD16 is included to further characterize NK cell subsets; the combination of CD16 and CD56 expression can identify the major NK cell population as early NK cells，Mature NK cells and Terminal NK cells. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
流式分析
Schematic diagram of Human NK Cell Panel Kit
Human peripheral blood cells were stained using the Human NK Cell Panel consisting of anti-human CD3 Alexa Fluor® 647, anti-human CD16 Alexa Fluor® 488 and anti-human CD56 PE conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the human peripheral blood cells excluding cellular debris.
2. Create a Fixable Viability Dye 777 (S0B88805) vs SSC-A dot plot with the human peripheral blood cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 Alexa Fluor® 647-A vs SSC-A dot plot and apply the CD45+ gate.
4. Create a CD16 Alexa Fluor® 488-A vs CD56 PE-A dot plot and apply the CD3- gate. Create gates to encompass CD16+ CD56+ cells (NK cells).
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