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反应种属 ?
Hu性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
| 货号 | 产品名称 | 推荐用量 | 规格 | 数量 |
|---|---|---|---|---|
| S0B5572-P | APC-Cy7 Mouse Anti-Human CD3 Antibody (HIT3a) | 5μl/T | 50T | 1 |
| S0B8156-P | Pacific Blue Mouse Anti-Human CD8 Antibody (S-3203) | 5μl/T | 50T | 1 |
| S0B1537-P | FITC Mouse Anti-Human CD4 Antibody (S-574-16) | 5μl/T | 50T | 1 |
| S0B8638-P | PE Mouse Anti-Human CD69 Antibody (FN50) | 5μl/T | 50T | 1 |
| S0B5942-P | PerCP-Cy5.5 Mouse Anti-Human CD103 Antibody (Ber-ACT8) | 5μl/T | 50T | 1 |
| S0B5140-P | APC Mouse Anti-Human CD62L Antibody (S-937-77) | 5μl/T | 50T | 1 |
This six‑color panel is designed for characterizing T‑cell subsets, with a focus on tissue‑resident memory T cells, in human peripheral blood or other biological samples. CD3 was included as a general T‑cell marker, and CD4 and CD8 were included to identify helper T‑cell and cytotoxic T‑cell subpopulations. CD62L is a marker often used to distinguish central memory T cells from effector and tissue‑resident subsets. Finally, the combined expression of CD69,CD103 and CD62L can identify canonical tissue‑resident memory T cells, non‑epithelial tissue‑resident T cells and central memory T cells. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
流式分析
Human PBMCs were stained using the Human Tissue-Resident T Cells Kit consisting of anti-human CD3 APC-Cy7, anti-human CD8 Pacific Blue, anti-human CD4 FITC, anti-human CD69 PE, anti-human CD103 PerCP-Cy5.5 and anti-human CD62L APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD4 FITC-A vs CD8 Pacific Blue-A dot plot and apply the CD3+ gate to identify the T cells subpopulations.
4. Create a CD62L APC-A vs SSC-A dot and apply the CD3+ gate.
5. Create a CD69 PE-A vs CD103 PerCP-Cy5.5-A dot and apply the CD62L+ gate. Create gates to encompass CD69- CD103- cells (Central memory T cells, TCM)
6. Create a CD69 PE-A vs CD103 PerCP-Cy5.5-A dot and apply the CD62L- gate. Create gates to encompass CD69+CD103+ cells (Canonical tissue-resident memory T cells, TRM), CD69+CD103- cells (Non-epithelial tissue-resident T cells)PHA stimulated Human PBMCs were stained using the Human Tissue-Resident T Cells Kit consisting of anti-human CD3 APC-Cy7, anti-human CD8 Pacific Blue, anti-human CD4 FITC, anti-human CD69 PE, anti-human CD103 PerCP-Cy5.5 and anti-human CD62L APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD4 FITC-A vs CD8 Pacific Blue-A dot plot and apply the CD3+ gate to identify the T cells subpopulations.
4. Create a CD62L APC-A vs SSC-A dot and apply the CD3+ gate.
5. Create a CD69 PE-A vs CD103 PerCP-Cy5.5-A dot and apply the CD62L+ gate. Create gates to encompass CD69- CD103- cells (Central memory T cells, TCM)
6. Create a CD69 PE-A vs CD103 PerCP-Cy5.5-A dot and apply the CD62L- gate. Create gates to encompass CD69+CD103+ cells (Canonical tissue-resident memory T cells, TRM), CD69+CD103- cells (Non-epithelial tissue-resident T cells)
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