分享产品介绍 评论(0)
反应种属 ?
Ms性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
| 货号 | 产品名称 | 推荐用量 | 规格 | 数量 |
|---|---|---|---|---|
| S0B5085-P | APC Armenian Hamster Anti-Mouse CD11c Antibody (N418) | 5μl/T | 50T | 1 |
| S0B1661-P | FITC Rat Anti-Mouse I-A/I-E Antibody (M5/114.15.2) | 5μl/T | 50T | 1 |
| S0B5160-P | PerCP-Cy5.5 Rat Anti-Mouse/Human CD11b Antibody (M1/70) | 5μl/T | 50T | 1 |
This three‑color panel is designed for identifying conventional dendritic cell subsets, namely cDC1 and cDC2 in mouse tissue cells. CD11c and I‑A/I‑E (MHC class II) were included to identify dendritic cells. CD11b was included to distinguish conventional type 1 dendritic cells(cDC1) from conventional type 2 dendritic cells(cDC2) within the dendritic cells. The panel is compatible with most two‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
流式分析
Mouse cutaneous cells were stained using the Mouse DC Panel Kit consisting of anti-mouse CD11c APC, anti-mouse Mouse I-A/I-E FITC, and anti-mouse CD11b PerCP-Cy5.5 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 583 (S0B88803) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD11b PerCP-Cy5.5-A vs SSC-A dot plot and apply the CD45+ gate.
4. Create a CD11c APC-A vs SSC-A dot plot and apply the CD11b+ gate. Create gates to encompass CD11b-CD11c+I-A/I-E+ cells (cDC1), CD11b+CD11c+I-A/I-E+ cells (cDC2).
评论(0)