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反应种属 ?
Ms性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
| 货号 | 产品名称 | 推荐用量 | 规格 | 数量 |
|---|---|---|---|---|
| S0B5627-P | Pacific Blue Rat Anti-Mouse CD45R/B220 Antibody (RA3-6B2) | 5μl/T | 50T | 1 |
| S0B5060-P | APC-Cy7 Rat Anti-Mouse CD19 Antibody (1D3) | 5μl/T | 50T | 1 |
| S0B1511-P | Alexa Fluor® 488 Mouse Anti-Mouse CD20 Antibody (18B12) | 5μl/T | 50T | 1 |
| S0B5132-P | PE-Cy7 Rat Anti-Mouse CD38 Antibody (90) | 5μl/T | 50T | 1 |
This four‑color panel is designed for detecting B cells and evaluating their differentiation or activation status in mouse tissue cells. CD19 and CD45R/B220 were included as general B‑cell markers to identify the total B‑cell population. CD20 was included as an additional marker for mature B cells. Finally, CD38 serves as a key marker to evaluate B cell activation and maturation stages, allowing for the differentiation between germinal center (GC) and non-germinal center B cells. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
流式分析
Mouse intestinal cells were stained using the Mouse B Cell panel Kit consisting of anti-mouse CD19 APC-Cy7, anti-mouse CD45R/B220 Pacific Blue, anti-mouse CD20 Alexa Fluor® 488 and anti-mouse CD38 PE-Cy7.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 583 (S0B88803) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD20 Alexa Fluor® 488-A vs SSC-A and CD45R/B220 Pacific Blue-A vs CD19 APC-Cy7-A dot plot show the B cells with the Fixable Viability Dye 583- gate applied.
4. Create a CD38 PE-Cy7-A vs SSC-A dot plot with the CD45R/B220+CD19+ gate applied.Mouse splenocytes were stained using the Mouse B Cell panel Kit consisting of anti-mouse CD19 APC-Cy7, anti-mouse CD45R/B220 Pacific Blue, anti-mouse CD20 Alexa Fluor® 488 and anti-mouse CD38 PE-Cy7.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the lymphocyte population.
2. Create a Fixable Viability Dye 583 (S0B88803) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD20 Alexa Fluor® 488-A vs SSC-A and CD45R/B220 Pacific Blue-A vs CD19 APC-Cy7-A dot plot show the B cells with the Fixable Viability Dye 583- gate applied.
4. Create a CD38 PE-Cy7-A vs SSC-A dot plot with the CD45R/B220+CD19+ gate applied.
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