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反应种属 ?
Hu性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
| 货号 | 产品名称 | 推荐用量 | 规格 | 数量 |
|---|---|---|---|---|
| S0B1527-P | FITC Mouse Anti-Human CD19 Antibody (S-1039-46) | 5μl/T | 50T | 1 |
| S0B5440-P | Pacific Blue Mouse Anti-Human CD20 Antibody (2H7) | 5μl/T | 50T | 1 |
| S0B5646-P | PE Mouse Anti-Human CD138 Antibody (MI15) | 5μl/T | 50T | 1 |
| S0B5334-P | Alexa Fluor® 647 Mouse Anti-Human CD24 Antibody (ML5) | 5μl/T | 50T | 1 |
| S0B5734-P | PE-Cy7 Mouse Anti-Human CD38 Antibody (S-R507-1) | 5μl/T | 50T | 1 |
This five‑color panel is designed for characterizing B‑cell subsets and plasma cells in human peripheral blood or other biological samples, with a focus on differentiation stages from immature to mature B lymphocytes and terminally differentiated antibody‑secreting cells. CD19 was included as a pan‑B‑cell marker to identify the total B‑cell population, and CD20 was included to distinguish mature B cells from plasma cells, as CD20 is downregulated upon terminal differentiation. CD138 is a marker often used to detect plasma cells; when combined with CD38, which is highly expressed on activated B cells and plasmablasts, the panel allows the discrimination of plasma cells, activated B cells/plasmablasts, and resting mature B cells. Furthermore, the expression pattern of CD38 and CD24 on B cells can identify transitional B cells, plasmablasts, and resting mature B cells. The panel is compatible with most standard three‑laser flow cytometers. If desired, the panel can serve as a backbone B‑cell tube, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest for more refined subsetting.
流式分析
Human PBMCs were stained using the Human B Cell panel consisting of anti-human CD19 FITC, anti-human CD20 Pacific Blue, anti-human CD138 PE, anti-human CD24 Alexa Fluor® 647 and anti-human CD38 PE-Cy7 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the leukocyte population.
2. Create a Fixable Viability Dye 777 (S0B88805) vs SSC-A dot plot with the leukocyte gate applied. Draw a gate around the live cell population.
3. Create a CD19 FITC-A vs CD20 Pacific Blue plot show the Mature B cells with the Fixable Viability Dye 545- gate applied.
4. Create a CD19 FITC-A vs CD24 Alexa Fluor® 647 dot show the immature B cells, epithelial cells and tissue-resident precursor cells with the Fixable Viability Dye 545- gate applied.
5. Create a CD138 PE-A vs CD38 PE-Cy7-A dot plot and apply the CD19- gate. Create gates to encompass CD138+CD38+ cells (Plasma cells), CD38+CD138- cells (Activated B cells /plasmablasts), and CD38-CD138- cells (Resting mature B cells)
6. Create a CD38 PE-Cy7-A vs CD24 Alexa Fluor® 647-A dot plot and apply the CD19+ gate. Create gates to encompass CD38+CD24+ cells (transitional B cells), CD38+CD24- cells (plasmablasts), and CD38-CD138- cells (Resting mature B cells)
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