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反应种属 ?
Ms性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
| 货号 | 产品名称 | 推荐用量 | 规格 | 数量 |
|---|---|---|---|---|
| S0B1692-P | APC-Cy7 Rat Anti-Mouse CD45 Antibody (30-F11) | 5μl/T | 50T | 1 |
| S0B1783-P | FITC Armenian hamster Anti-Mouse CD3 Antibody (S-R491) | 5μl/T | 50T | 1 |
| S0B5751-P | APC Rat Anti-Mouse CD49b Antibody (DX5) | 5μl/T | 50T | 1 |
This three‑color panel is designed for detecting NK cells and NKT cells in mouse tissue cells. CD45 serves as a pan-leukocyte marker, while CD3 defines total T cells. CD49b is incorporated as a canonical marker widely used to detect natural killer (NK) cells. The panel is compatible with most two‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
流式分析
Mouse hepatocytes were stained using the Mouse NK Cell Panel consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD3 FITC and anti-mouse CD49b APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs CD49b APC A dot plot and apply the CD45+ gate. Create gates to encompass CD3+CD49b+ cells (NK-T cells), CD3-CD49b+ cells (NK cells)Mouse pulmonary cells were stained using the Mouse NK Cell Panel consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD3 FITC and anti-mouse CD49b APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs CD49b APC A dot plot and apply the CD45+ gate. Create gates to encompass CD3+CD49b+ cells (NK-T cells), CD3-CD49b+ cells (NK cells)Mouse cutaneous cells were stained using the Mouse NK Cell Panel consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD3 FITC and anti-mouse CD49b APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs CD49b APC A dot plot and apply the CD45+ gate. Create gates to encompass CD3+CD49b+ cells (NK-T cells), CD3-CD49b+ cells (NK cells)Mouse splenocytes were stained using the Mouse NK Cell Panel consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD3 FITC and anti-mouse CD49b APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs CD49b APC A dot plot and apply the CD45+ gate. Create gates to encompass CD3+CD49b+ cells (NK-T cells), CD3-CD49b+ cells (NK cells)Mouse intestinal cells were stained using the Mouse NK Cell Panel consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD3 FITC and anti-mouse CD49b APC conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 452 (S0B88801) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD3 FITC-A vs CD49b APC A dot plot and apply the CD45+ gate. Create gates to encompass CD3+CD49b+ cells (NK-T cells), CD3-CD49b+ cells (NK cells)
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