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反应种属 ?
Ms性状
Liquid缓冲体系
PBS, 1% BSA, 0.09% sodium azide
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied.
应用
FCM
| 货号 | 产品名称 | 推荐用量 | 规格 | 数量 |
|---|---|---|---|---|
| S0B1692-P | APC-Cy7 Rat Anti-Mouse CD45 Antibody (30-F11) | 5μl/T | 50T | 1 |
| S0B5160-P | PerCP-Cy5.5 Rat Anti-Mouse/Human CD11b Antibody (M1/70) | 5μl/T | 50T | 1 |
| S0B5458-P | PE Rat Anti-Mouse F4/80 Antibody (S-R537) | 5μl/T | 50T | 1 |
| S0B1673-P | Alexa Fluor® 488 Rat Anti-Mouse CD86/B7-2 Antibody (GL-1) | 5μl/T | 50T | 1 |
| S0B5331-P | Alexa Fluor® 647 Rat Anti-mouse CD206 (MMR) Antibody (S-R498) | 5μl/T | 50T | 1 |
| S0B1700-P | Alexa Fluor® 700 Rat Anti-Mouse Ly6G Antibody (1A8) | 5μl/T | 50T | 1 |
This six‑color panel is designed for identifying macrophage populations and evaluating their polarization status in mouse tissue cells. CD45 is included as a general leukocyte marker to gate all hematopoietic cells. CD11b is included as a myeloid cell marker, and F4/80 is included as a macrophage‑specific marker. Ly6G is used to exclude granulocytes. Finally, the expression of CD86 and CD206 on the gated macrophage population can distinguish M1 and M2 polarized subsets, respectively. The panel is compatible with most three‑laser flow cytometers. If desired, the panel can serve as a backbone panel, allowing for the addition of antibodies conjugated to various fluorophores to detect other markers of interest.
流式分析
Schematic diagram of Mouse MacroPhage Panel Kit (CD45/CD11b/F4/80/Ly6G/CD206/CD86)
Mouse hepatocytes were stained using the Mouse MacroPhage Panel Kit consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD11b PerCP-Cy5.5, anti-mouse F4/80 PE, anti-mouse CD86 Alexa Fluor® 488, anti-mouse CD206 Alexa Fluor® 647 and anti-mouse LY6G Alexa Fluor® 700 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD11b PerCP-Cy5.5-A vs SSC-A dot plot and apply the CD45+ gate.
4. Create a Ly6G Alexa Fluor® 700-A vs F4/80 PE-A dot plot and apply the CD11b+ gate. Create gates to encompass F4/80+Ly6G- cells (MacroPhage)
5. Create a CD86 Alexa Fluor® 488-A vs SSC-A plot show the M1 MacroPhage with the F4/80+Ly6G- gate applied.
6. Create a CD206 Alexa Fluor® 647-A vs SSC-A dot show the M2 MacroPhage with the F4/80+Ly6G- gate applied.Mouse pulmonary cells were stained using the Mouse MacroPhage Panel Kit consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD11b PerCP-Cy5.5, anti-mouse F4/80 PE, anti-mouse CD86 Alexa Fluor® 488, anti-mouse CD206 Alexa Fluor® 647 and anti-mouse LY6G Alexa Fluor® 700 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD11b PerCP-Cy5.5-A vs SSC-A dot plot and apply the CD45+ gate.
4. Create a Ly6G Alexa Fluor® 700-A vs F4/80 PE-A dot plot and apply the CD11b+ gate. Create gates to encompass F4/80+Ly6G- cells (MacroPhage)
5. Create a CD86 Alexa Fluor® 488-A vs SSC-A plot show the M1 MacroPhage with the F4/80+Ly6G- gate applied.
6. Create a CD206 Alexa Fluor® 647-A vs SSC-A dot show the M2 MacroPhage with the F4/80+Ly6G- gate applied.Mouse peritoneal exudates cells were stained using the Mouse MacroPhage Panel Kit consisting of anti-mouse CD45 APC-Cy7, anti-mouse CD11b PerCP-Cy5.5, anti-mouse F4/80 PE, anti-mouse CD86 Alexa Fluor® 488, anti-mouse CD206 Alexa Fluor® 647 and anti-mouse LY6G Alexa Fluor® 700 conjugated antibodies.
To analyze the data:
1. Create an FSC-A vs SSC-A dot plot and draw a gate around the tissue cells excluding cellular debris.
2. Create a Fixable Viability Dye 545 (S0B88802) vs SSC-A dot plot with the tissue cells gate applied. Draw a gate around the live cell population.
3. Create a CD11b PerCP-Cy5.5-A vs SSC-A dot plot and apply the CD45+ gate.
4. Create a Ly6G Alexa Fluor® 700-A vs F4/80 PE-A dot plot and apply the CD11b+ gate. Create gates to encompass F4/80+Ly6G- cells (MacroPhage)
5. Create a CD86 Alexa Fluor® 488-A vs SSC-A plot show the M1 MacroPhage with the F4/80+Ly6G- gate applied.
6. Create a CD206 Alexa Fluor® 647-A vs SSC-A dot show the M2 MacroPhage with the F4/80+Ly6G- gate applied.
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