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宿主来源
Human分子别名
Anti-human CD16/CD32/CD64，Fc Blocker, FcγR blocker, Fc inhibitor反应种属 ?
Hu预测反应种属
(反应种属缩写表)Human浓度
3 mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
Fc receptor blocking
稀释度
应用 稀释度 Fc receptor blocking 3μg (1μl/test) per million cells in 100μl volume
FcR Blocking Reagent is a specialized reagent used in flow cytometry experiments to prevent non-specific binding between the Fc segment of antibodies and Fc receptors (FcRs) on the cell surface. This is crucial to avoid false positive or negative results in immunofluorescence staining.
If the customer uses the detection antibody with human species after the blocking step, please make sure to use the labeled primary antibody.
流式分析
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labeling Rabbit monoclonal IgG Isotype after incubated for 30 min on ice in PBS (Left) compared with Human FcR Blocking Reagent at 3 µg/test (Right) to block Fc receptors and non-specific protein . Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labeling Rabbit monoclonal IgG Isotype after incubated for 30 min on ice in PBS (Black) compared with Human FcR Blocking Reagent at 3 µg/test (Red) to block Fc receptors and non-specific protein and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
多重免疫组化
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labeling Rabbit monoclonal IgG Isotype after incubated for 30 min on ice in PBS (Black) compared with Human FcR Blocking Reagent at 1/300 (1 µg, 1x10^6 in 100 μL) dilution (Red) to block Fc receptors and non-specific protein and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
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