分享产品介绍 引用文献(1) 评论(0)
分子别名
Anti-human CD16/CD32/CD64反应种属 ?
Hu浓度
3mg/ml标记
Unconjugated性状
Liquid缓冲体系
PBS pH7.4, 0.03% Proclin 300
储存条件
12 months from date of receipt / reconstitution, 2 to 8 °C as supplied
应用
Fc receptor blocking
稀释度
应用 稀释度 Fc receptor blocking 3μg (1μl/test) per million cells in 100μl volume
FcR Blocking Reagent is a specialized reagent used in flow cytometry experiments to prevent non-specific binding between the Fc segment of antibodies and Fc receptors (FcRs) on the cell surface. This is crucial to avoid false positive or negative results in immunofluorescence staining.
组分和操作说明
-
If the customer uses the detection antibody with mouse species after the blocking step, please make sure to use the labeled primary antibody.
-
流式分析
Flow cytometric analysis of human PBMC (human peripheral blood mononuclear cell) labeling Rabbit monoclonal IgG Isotype after incubated for 30 min on ice in PBS (Left) compared with Human FcR Blocking Reagent at 3 µg/test (Right) to block Fc receptors and non-specific protein. Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
Flow cytometric analysis of THP-1 (Human monocytic leukemia monocyte) labeling Rabbit monoclonal IgG Isotype after incubated for 30 min on ice in PBS (Black) compared with Human FcR Blocking Reagent at 3 µg/test (Red) to block Fc receptors and non-specific protein and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
引用文献(1)
- IL-35 alleviates ferroptosis in macrophage by activating the NRF2/GPX4 pathway to improve sepsis-induced ARDS
P Liu, C Zhang, M Guo, S Ai, Y Zhao, R Luo, F Xu
Cytokine. 2025 Dec 09 .
影响因子： 3.7
货号：S0F0002产品名称：Human FcR Blocking Reagent (Mouse anti-human CD16/32 & human antibody against CD64)
评论(0)