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克隆号
mAb014应用
ELISA反应种属 ?
Species Independent浓度
Lot specific* (generally 1 to 5 mg/ml)*纯度
99.4%标记
Unconjugated性状
Liquid缓冲体系
1*PBS,pH7.4储存条件
Store at -80ºC. Avoid multiple freeze-thaw cycles.
电泳
Fig.1. SDS-PAGE analysis of Eribulin_mAb014
Lane M: Precision Plus Protein™ Dual Color Standards (Bio-Rad) (Sample loaded: 5 μl)
Lane 1: Purified Eribulin_mAb014 under reducing condition (Sample loaded: 2 μg)
Lane 2: Purified Eribulin_mAb014 under non-reducing condition (Sample loaded: 2 μg)
• Recombinant protein was visualized by Coomassie Brilliant Blue R250 staining.
体积排阻色谱(SEC-HPLC)
Fig. 2. SEC-HPLC analysis of Eribulin_mAb014
Running buffer: 2*PBS, pH 7.4
Column: Superdex 200 Increase 5/150 GL(GE, Code Number: 28-9909-45)
ELISA
Fig.3. ELISA analysis of Eribulin mAb014
Coating Eribulin ADC: 1ug/ml
Add anti-Eribulin mAb: from 5ug/ml, 10-fold diluent, 8 points including 0
Add Anti-mouse IgG (Fc specific)-HRP: 1:5000
Fig.4. Sandwich ELISA analysis of Eribulin mAb014
Coating Her2 ECD protein: 2ug/ml
Add Eribulin-ADC: from 10ug/ml, 10-fold diluent, 8 points including 0
Add eribulin antibody mAb014 (mouse IgG) at 1ug/ml
Add anti-mouse IgG (Fc specific)-HRP: 1:5000
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