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稀释度
储存条件
12 months from date of receipt, -20 °C as supplied
Fixable Viability Dye 740 is excited by the Red laser (with an excitation maximum of 615 nm) and has fluorescence emission maximum at 740 nm, Fixable Viability Dye 740 has similar emission to Alexa Fluor® 700. Fixable Viability Dye 740 can intracellular diffusion of the dye and covalent binding to higher overall concentrations of amines, Each lot of this product is quality control tested, is useful for discrimination of viable from non-viable mammalian cells in multicolor flow cytometric applications. Each lot of this product is quality control tested. The labeled cells can be fixed with formaldehyde for downstream decontamination, freezing and/or permeabilization and subsequent intracellular staining while maintaining stable viability stain fluorescence.
组分和操作说明
For reconstitution, pre-warm the kit to room temperature; add 100 µL of DMSO to one vial of Fixable Viability Dye 740 and mix until fully dissolved. After reconstitution, store the stock solution at -20°C in small aliquots. Use up within 3 months. Repeated freezing and thawing is not recommended.
For recommended usage, add 1 µl of Fixable Viability Dye 740 Stock Solution for each 2~5 ml of cell suspension/PBS (1:2000~1:5000) and vortex immediately. It is recommended that the reagent be titrated for optimal performance for each application.
Please refer to the protocol for details.
流式分析
Mouse splenocytes were stained with Fixable Viability Dye 740 and analyzed before fixation (Middle Panels) or after fixation (Right Panels). Cells alone, without Fixable Viability Dye 740 staining (Left Panels). Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
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