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Goat性状
Liquid储存条件
12 months from date of receipt / reconstitution, -20 °C as supplied;
2 to 8 °C for 1 week under working solution.应用
Blocking ,IHC-P ? ,ICC ? ,IF ?
This product is used for the blocking of non-specific antibody binding in tissue and cell staining. According to experimental requirements, use PBS (pH7.4) solution for 10 times dilution.
免疫组化
IHC shows positive staining in paraffin-embedded human colon. Anti-MUC1 antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Slides were blocked with 10% normal goat serum for 1 hour (RT).
免疫细胞化学
ICC shows positive staining in MCF7 cells. Anti-Claudin 4 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
Slides were blocked with 10% normal goat serum for 1 hour (RT).
免疫荧光
IF shows positive staining in paraffin-embedded human thyroid cancer. Anti-CK-LMW antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/500 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
Slides were blocked with 10% normal goat serum for 1 hour (RT).
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