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产品中心 ELISA试剂盒 即用型ELISA试剂盒

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Human IL-6 (Culture Media) HS ELISA Kit

价格 ￥4,000.00 （ 1-2周）
货号 S0C9001
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-6

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3.4%；
      Inter-assay: 4.0%

    • Sample type

      Cell culture supernatant

    • Assay type

      Sandwich(quantitative)

    • Sensitivity

      0.0421pg/ml

    • Range

      0.16pg/mL – 10pg/mL

    • Recovery

      Cell culture supernatant: 99%

    • Assay time

      2.5 hours

    • Species reactivity

      Human
背景介绍

  • Interleukin 6 (IL-6) is a prototypical cytokine for maintaining homeostasis. When homeostasis is disrupted by infections or tissue injuries, IL-6 is produced immediately and contributes to host defense against such emergent stress through activation of acute-phase and immune responses. However, dysregulated excessive and persistent synthesis of IL-6 has a pathological effect on, respectively, acute systemic inflammatory response syndrome and chronic immune-mediated diseases.

  • ELISA

    • Standard curve
      Example of Human IL-6 standard curve in Assay Diluent #1.

    • Linearity
      The concentrations of IL-6 were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (0.5%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 88,750 pg/mL in stimulated human PBMC supernatant.
      #2 sample is undiluted samples are as follows: human PBMC cells culture for 5days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 46 pg/mL in unstimulated human PBMC supernatant.

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