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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Monkey IL-10 OneStep ELISA Kit

价格 ￥4,000.00 （ 1-2周）
货号 S0C3204
规格
数量
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IL-10

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Mk

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 5.8%
      Inter-assay: 6.8%

    • Sample type

      Cell culture supernatant; Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.055 ng/mL

    • Range

      0.156 ng/mL – 10 ng/mL

    • Recovery

      Cell culture supernatant: 88%
      Monkey Serum: 96%
      EDTA Plasma: 99%

    • Assay time

      60 minutes

    • Species reactivity

      Hu
背景介绍

  • Monkey Interleukin-10 (IL-10) is a crucial anti-inflammatory cytokine that plays a central role in regulating immune responses and maintaining immune homeostasis. It is produced by various immune cells, including regulatory T cells, macrophages, and dendritic cells, to limit excessive tissue disruption caused by inflammation. The protein exerts its effects by binding to a heterotetrameric receptor complex consisting of IL-10R alpha and IL-10R beta, which activates the JAK/STAT3 signaling pathway. This signaling cascade subsequently suppresses the production of pro-inflammatory cytokines like TNF-α, IL-1β, and IFN-γ, and also reduces the expression of MHC class II molecules on antigen-presenting cells. Recombinant forms of the monkey IL-10 protein, typically derived from cynomolgus or rhesus macaques, are commonly produced in various expression systems such as yeast, E. coli, and HEK293 cells for research purposes. These recombinant proteins, which are approximately 160 amino acids in length with a molecular weight around 18.7 kDa, are essential tools for studying IL-10 signaling and immune modulation in non-human primate models. Due to the high genetic and immunological similarity between monkeys and humans, research on monkey IL-10 provides critical translational insights into human infectious, autoimmune, and inflammatory diseases.

  • ELISA

    • Standard curve
      Example of Monkey IL-10 standard curve in Assay Diluent G1.

    • Dilution linearity
      The concentrations of Monkey IL-10 were measured and interpolated from the target standard curves and corrected for sample dilution.
      The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (100%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 6.8 ng/mL in PBMC stimulated supernatant.

    • Spike Recovery
      The recovery of Monkey IL-10 was evaluated in activated samples spiked with concentrations spanning the entire assay range.

    • Spike-and-dilution Linearity
      To evaluate assay linearity, three samples were spiked with high levels of Monkey IL-10 in different matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.

    • Intra-Assay Precision (Precision within an assay)
      Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
      Inter-Assay Precision (Precision between assays).
      Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.

    • Determination of Minimum Detectable Dose (MDD)
      The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
      MDD = 2 × STDEV + AVERAGE

    • Cross-reactivity
      The cross-reactivity among Mouse IL-10, Monkey IL-10, and Human IL-10 was evaluated by testing them in the same assay system. Serial dilutions of each protein were measured, and the dose-response curves were compared. When Mouse IL-10 was tested in the Monkey IL-10 assay, no significant cross-reactivity was observed, and the signal remained within the background range of the assay. When Human IL-10 was tested in the Monkey IL-10 assay, significant cross-reactivity was observed.

    • HOOK Effect Threshold
      The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.

    • Protocol Diagram

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