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免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Mk交叉反应
Hu
纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8°C as supplied.
Precision
Intra-assay: 3.9%；
Inter-assay: 4.1%Sample type
Serum; Heparin Plasma, Citrate Plasma; Cell Culture SupernatantAssay type
Sandwich (quantitative)Sensitivity
9.75 pg/mLRange
23.44 pg/mL – 1500 pg/mLRecovery
Serum: 101%
Citrate plasma: 93%
Heparin Plasma: 94%
Cell Culture Supernatant: 112%Assay time
60 minutesSpecies reactivity
MkCROSS REACTIVITY
Hu
Monkey E-selectin (CD62E, ELAM-1) is a 107–115 kDa type I transmembrane glycoprotein of the selectin family, structurally composed of an extracellular C-type lectin domain (responsible for calcium-dependent carbohydrate recognition), an EGF-like domain, and six short consensus repeats, followed by a transmembrane region and a short cytoplasmic tail. Cynomolgus monkey E-selectin shares 96% amino acid identity with human E-selectin, making it a highly relevant translational model. E-selectin is expressed exclusively on activated endothelial cells, with peak induction at approximately 4 hours after stimulation by pro-inflammatory cytokines (TNF-α, IL-1β), followed by rapid decline within 24 hours. It mediates the initial tethering and rolling of leukocytes (neutrophils, monocytes, memory T cells) along the vascular endothelium by binding to sialylated/fucosylated ligands such as Sialyl Lewis X (CD15s) and PSGL-1 (CD162). Key binding residues identified by crystallography (PDB 1g1t) include Y48, N82, N83, E92, Y94, R97, N105, E107, E80, E98, and D106. After peak expression, E-selectin is proteolytically cleaved into soluble E-selectin, which circulates in blood and serves as a biomarker of endothelial activation.
ELISA
Standard Curve
Example of Monkey E-selectin standard curve in Assay Diluent A1. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.Spike Recovery
The recovery of Monkey E-Selectin was assessed by spiking a standard into serum or plasma. Serial dilutions of the neat biological sample were prepared, and the percent recovery was calculated for each dilution relative to the expected concentration.Spike Recovery
The recovery of Monkey E-Selectin was evaluated in medium spiked with concentrations spanning the entire assay range.Dilution linearity
The concentrations of Monkey E-Selectin were measured and interpolated from the target standard curves and corrected for sample dilution.
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (1%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 116135.5 pg/mL in monkey serum.Dilution linearity
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (5%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 21254.3 pg/mL in monkey heparin plasma.Dilution linearity
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (5%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 23145.2 pg/mL in monkey citrate plasma.Spike-and-dilution Linearity
To evaluate assay linearity, Cell culture media was spiked with high levels of Monkey E-selectin in matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.Spike-and-dilution Linearity
To evaluate assay linearity, native E-selectin was measured in monkey serum and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.Spike-and-dilution Linearity
To evaluate assay linearity, native E-selectin was measured in monkey plasma and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGEHOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.Protocol Diagram
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