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抗原名称
Human E-selectin/CD62E免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu交叉反应
Mk
纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8°C as supplied.
Precision
Intra-assay: 4.0%；
Inter-assay: 4.5%Sample type
Serum; EDTA Plasma, Heparin Plasma, Citrate Plasma; Cell Culture SupernatantAssay type
Sandwich (quantitative)Sensitivity
6.12 pg/mLRange
15.63 pg/mL – 1000 pg/mLRecovery
Serum: 94%
EDTA Plasma: 97%
Citrate plasma: 98%
Heparin Plasma: 100%
Cell Culture Supernatant: 100%Assay time
60 minutesSpecies reactivity
HuCROSS REACTIVITY
Mk
E-selectin (also known as CD62E, ELAM-1, or SELE) is a type I transmembrane glycoprotein belonging to the selectin family of cell adhesion molecules. It is exclusively expressed on activated vascular endothelial cells in response to inflammatory cytokines such as IL-1β and TNF-α. Unlike P-selectin, which is pre-stored in Weibel-Palade bodies, E-selectin requires de novo gene transcription and protein synthesis. It is a cell adhesion glycoprotein exclusively expressed on activated vascular endothelial cells during inflammation, mediating the initial rolling and tethering of leukocytes via calcium-dependent recognition of sialyl Lewis X/Lewis A glycans. Beyond its central role in leukocyte trafficking, E-selectin is implicated in tumor metastasis, angiogenesis, and immune regulation. Soluble E-selectin serves as a clinical biomarker for endothelial activation in cardiovascular disease, sepsis, and autoimmune disorders. Consequently, E-selectin proteins are indispensable tools for both fundamental mechanistic studies and the development of diagnostic or therapeutic strategies targeting pathological inflammation and cancer spread.
ELISA
Standard Curve
Example of Human E-selectin standard curve in Assay Diluent A1. Plotted are the background-subtracted data.
This standard curve is provided for demonstration only.Spike Recovery
The recovery of Human E-Selectin was assessed by spiking a standard into serum or plasma. Serial dilutions of the neat biological sample were prepared, and the percent recovery was calculated for each dilution relative to the expected concentration.Spike Recovery
The recovery of Human E-Selectin was evaluated in medium spiked with concentrations spanning the entire assay range.Dilution linearity
The concentrations of Human E-Selectin were measured and interpolated from the target standard curves and corrected for sample dilution.
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (2.22%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 39606.7 pg/mL in human serum.Dilution linearity
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (1.11%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 72969.0 pg/mL in human EDTA plasma.Dilution linearity
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (1%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 77374.1 pg/mL in human heparin plasma.Dilution linearity
The leftmost bar in the graph represents neat biological samples. "Undiluted" was defined as the neat biological sample (1.54%). The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 46169.3 pg/mL in human citrate plasma.Spike-and-dilution Linearity
To evaluate assay linearity, Cell culture media was spiked with high levels of Human E-selectin in matrices and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.Spike-and-dilution Linearity
To evaluate assay linearity, native E-selectin was measured in human serum and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.Spike-and-dilution Linearity
To evaluate assay linearity, native E-selectin was measured in human EDTA plasma and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.Spike-and-dilution Linearity
To evaluate assay linearity, native E-selectin was measured in human heparin plasma and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.Spike-and-dilution Linearity
To evaluate assay linearity, native E-selectin was measured in human citrate plasma and serially diluted with the corresponding Calibrator Diluent to fall within the assay's dynamic range.Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays).
Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.Determination of Minimum Detectable Dose (MDD)
The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
MDD = 2 × STDEV + AVERAGEHOOK Effect Threshold
The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.Protocol Diagram
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