Human MPO OneStep ELISA Kit

Datasheet COA

价格￥3,000.00 （ 1-2周）

货号 S0C3188

规格

1x96T5x96T10x96T

数量

产品介绍

产品介绍评论(0)

产品规格

抗原名称

MPO
免疫原

Recombinant Protein
抗体类型

Recombinant mAb
反应种属 ?

Hu
纯化方式

Protein A
储存条件

12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

- Precision
  
  Intra-assay: 3.3%；
  Inter-assay: 4.7%
- Sample type
  
  Serum; EDTA Plasma, Heparin Plasma, Citrate Plasma; Cell Culture Supernatant
- Assay type
  
  Sandwich (quantitative)
- Sensitivity
  
  5.13 pg/mL
- Range
  
  23.44 pg/mL – 1500 pg/mL
- Recovery
  
  Serum: 93%
  EDTA Plasma: 99%
  Citrate plasma: 98%
  Heparin Plasma: 103%
  Cell Culture Supernatant: 84%
- Assay time
  
  60 minutes
- Species reactivity
  
  Hu
- CROSS REACTIVITY
  
  Monkey, Hamster, Rat, Cat

背景介绍

Myeloperoxidase (MPO) is a heme-containing peroxidase enzyme predominantly expressed in neutrophil granulocytes, where it constitutes approximately 5% of the cell's dry weight, and plays an essential role in innate immunity by catalyzing the conversion of hydrogen peroxide and chloride ions into hypochlorous acid—a potent antimicrobial agent. Structurally, the mature MPO is a tetramer composed of two light and two heavy chains, with a covalently bound heme cofactor critical for its catalytic activity. While MPO is vital for host defense against pathogens, its oxidative products can also damage host tissues, linking the enzyme to various inflammatory and non-infectious diseases. Clinically, MPO serves as a valuable biomarker for cardiovascular disease, where elevated levels predict coronary artery disease, vulnerable atherosclerotic plaques, and higher mortality in acute coronary syndrome patients; it also shows promise in emergency triage for chest pain. Additionally, MPO has been implicated in Alzheimer's disease, multiple sclerosis, lung cancer, and MPO deficiency—a genetic immune disorder. Collectively, MPO is a multifaceted protein with critical roles in host defense and disease pathogenesis, and its applications span biomarker discovery, cancer prognosis, hematological diagnostics, research tools, and therapeutic development.

ELISA
- Standard Curve
  Example of Human MPO standard curve in Assay Diluent A1. Plotted are the background-subtracted data.
  This standard curve is provided for demonstration only.
- Intra-Assay Precision (Precision within an assay)
  Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
  Inter-Assay Precision (Precision between assays).
  Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.
- Determination of Minimum Detectable Dose (MDD)
  The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
  MDD = 2 × STDEV + AVERAGE
- Cross-reactivity
  The cross-reactivity between Human MPO and Mouse MPO was assessed by testing both proteins in the same assay system. Serial dilutions of each protein were measured, and the dose-response curves were compared. No significant cross-reactivity was observed when Mouse MPO was tested in the Human MPO assay, as the signals remained within the background range of the assay.
- HOOK Effect Threshold
  The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.
- Protocol Diagram