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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Mouse IgM OneStep ELISA Kit

价格 ￥3,000.00 （ 1-2周）
货号 S0C3184
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IgM

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Ms

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 4.5%；
      Inter-assay: 5.1%

    • Sample type

      Serum; EDTA plasma; Cell Culture Supernatant

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.046 ng/mL

    • Range

      0.078 ng/mL – 5 ng/mL

    • Recovery

      Serum (Balb/c): 114%
      EDTA plasma (Balb/c): 107%
      Cell Culture Supernatant: 98%

    • Assay time

      60 minutes

    • Species reactivity

      Ms
背景介绍

  • Mouse immunoglobulin M (IgM) is a crucial component of the humoral immune system and is the first antibody produced by B-cells in response to an antigenic challenge. It exists in two forms: a pentameric (19S) form found in serum and a monomeric (8S) form on the surface of lymphocytes. The IgM pentamer, which is the predominant form in circulation, consists of five monomeric units and has a molecular weight of approximately 900 kDa, while the hexameric form has a molecular weight of about 1050 kDa. Each monomer of IgM is composed of two heavy chains and two light chains, with the heavy chain having four constant domains (CH1-CH4) and the light chain having one constant domain (CL). The CH4 domain facilitates oligomerization, leading to the formation of penta- or hexamers, and these oligomers are covalently linked via disulfide bonds, resembling a snowflake structure. A joining (J) chain, a 15 kDa polypeptide, is essential for the secretion of IgM into mucosal areas.

  • ELISA

    • Standard Curve
      Example of Mouse IgM standard curve in Assay Diluent A1. Plotted are the background-subtracted data.
      This standard curve is provided for demonstration only.

    • Spike Recovery
      Recovery of Mouse IgM was assessed by spiking a 5 ng/mL standard into 0.00063% serum. Recovery of Mouse IgM was assessed by spiking a 5 ng/mL standard into 0.00063% EDTA-Plasma. Serial dilutions of the spiked sample were prepared, and the percent recovery was calculated for each dilution relative to the expected concentration.

    • Dilution Linearity
      The concentrations of Mouse IgM were measured and interpolated from the target standard curves and corrected for sample dilution.
      The leftmost bar in the graph shows biological samples diluted in Assay Diluent to 0.0025% concentration. "undiluted" was defined as 0.0025% biological sample. The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 533.31 ug/mL in Balb/c Mouse serum.
      The mean target concentration was determined to be 285.11 ug/mL in Balb/c Mouse Citrate-plasma.
      The mean target concentration was determined to be 401.89 ug/mL in Balb/c Mouse EDTA-plasma.

    • Dilution Linearity
      The concentrations of Mouse IgM were measured and interpolated from the target standard curves and corrected for sample dilution.
      The leftmost bar in the graph shows biological samples diluted in Assay Diluent to 0.005% concentration. "undiluted" was defined as 0.005% biological sample. The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 140.21 ug/mL in C57 Mouse serum.
      The mean target concentration was determined to be 108.18 ug/mL in C57 Mouse Citrate-plasma.
      The mean target concentration was determined to be 125.21 ug/mL in C57 Mouse Heparin-plasma.

    • Dilution Linearity
      The concentrations of Mouse IgM were measured and interpolated from the target standard curves and corrected for sample dilution.
      The leftmost bar in the graph shows biological samples diluted in Assay Diluent to 0.005% concentration. "undiluted" was defined as 0.005% biological sample. The subsequent bars represent two-fold serial dilutions of this reference. The mean target concentration was determined to be 128.53 ug/mL in CD-1 Mouse serum.
      The mean target concentration was determined to be 117.52 ug/mL in CD-1 Mouse EDTA-plasma.
      The mean target concentration was determined to be 100.28 ug/mL in CD-1 Mouse Heparin-plasma.

    • Intra-Assay Precision (Precision within an assay)
      Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
      Inter-Assay Precision (Precision between assays).
      Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.

    • Determination of Minimum Detectable Dose (MDD)
      The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
      MDD = 2 × STDEV + AVERAGE

    • Cross‑reactivity
      Tests for the specificity of various serum and proteins showed no cross-reactivity.

    • HOOK Effect Threshold
      The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.

    • Protocol Diagram

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