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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Rat IgE Culture media OneStep ELISA Kit

价格 ￥3,000.00 （ 1-2周）
货号 S0C3183
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IgE

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Rt

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 2.6%
      Inter-assay: 4.2%

    • Sample type

      Cell culture supernatant

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.014 ng/mL

    • Range

      0.08 ng/mL – 5 ng/mL

    • Recovery

      Cell culture supernatant (1640): 94%
      Cell culture supernatant (DMEM): 96%

    • Assay time

      60 minutes
背景介绍

  • Immunoglobulin E (IgE) is a type of antibody (or immunoglobulin "isotype") that has been found only in mammals. IgE is synthesised by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing four Ig-like constant domains (Cε1–Cε4). IgE is thought to be an important part of the immune response against infection by certain parasitic worms, including Schistosoma mansoni, Trichinella spiralis, and Fasciola hepatica. IgE is also utilized during immune defense against certain protozoan parasites such as Plasmodium falciparum. IgE may have evolved as a defense to protect against venoms. IgE also has an essential role in type I hypersensitivity, which manifests in various allergic diseases, such as allergic asthma, most types of sinusitis, allergic rhinitis, food allergies, and specific types of chronic urticaria and atopic dermatitis. IgE also plays a pivotal role in responses to allergens, such as: anaphylactic reactions to drugs, bee stings, and antigen preparations used in desensitization immunotherapy.

  • ELISA

    • Standard curve
      Example of Rat IgE standard curve in Assay Diluent M1.

    • Spike Recovery
      The recovery of Rat IgE was evaluated in activated samples spiked with concentrations spanning the entire assay range.

    • Intra-Assay Precision (Precision within an assay)
      Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
      Inter-Assay Precision (Precision between assays)
      Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.

    • Determination of Minimum Detectable Dose (MDD)
      The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
      MDD = 2 × STDEV + AVERAGE

    • HOOK Effect Threshold
      The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.

    • Leading Competitor comparison

    • Protocol Diagram

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