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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Rat IgE Serum & Plasma OneStep ELISA Kit

价格 ￥3,000.00 （ 1-2周）
货号 S0C3182
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IgE

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Rt

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 1.9%
      Inter-assay: 3.1%

    • Sample type

      Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      0.05 ng/mL

    • Range

      0.16 ng/mL – 10 ng/mL

    • Recovery

      Rat SD Serum: 100%
      Rat SD EDTA Plasma: 98%
      Rat SD Heparin Plasma: 102%
      Rat SD Citrate Plasma: 103%
      Rat SHR Serum: 99%
      Rat SHR EDTA Plasma: 95%
      Rat Lewis Serum: 97%
      Rat Lewis EDTA Plasma: 93%

    • Assay time

      60 minutes
背景介绍

  • Immunoglobulin E (IgE) is a type of antibody (or immunoglobulin "isotype") that has been found only in mammals. IgE is synthesised by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing four Ig-like constant domains (Cε1–Cε4). IgE is thought to be an important part of the immune response against infection by certain parasitic worms, including Schistosoma mansoni, Trichinella spiralis, and Fasciola hepatica. IgE is also utilized during immune defense against certain protozoan parasites such as Plasmodium falciparum. IgE may have evolved as a defense to protect against venoms. IgE also has an essential role in type I hypersensitivity, which manifests in various allergic diseases, such as allergic asthma, most types of sinusitis, allergic rhinitis, food allergies, and specific types of chronic urticaria and atopic dermatitis. IgE also plays a pivotal role in responses to allergens, such as: anaphylactic reactions to drugs, bee stings, and antigen preparations used in desensitization immunotherapy.

  • ELISA

    • Standard curve
      Example of Rat IgE standard curve in Assay Diluent E1.

    • Linearity
      The concentrations of Rat IgE were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted (5.0%) samples are as follows: SD Rat serum was two-fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 109.8 ng/mL in SD Rat serum.

    • #2 sample is undiluted (10.0%) samples are as follows: SD Rat EDTA plasma was two-fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 32.0 ng/mL in SD Rat EDTA plasma.

    • #3 sample is undiluted (10.0%) samples are as follows: SD Rat Heparin plasma was two-fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 43.4 ng/mL in SD Rat Heparin plasma.

    • #4 sample is undiluted (5.0%) samples are as follows: Wistar Rat Citrate plasma was two-fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 33.3 ng/mL in Wistar Rat Citrate plasma.

    • Spike Recovery
      The recovery of Rat IgE was evaluated in activated samples spiked with concentrations spanning the entire assay range.

    • Specificity
      Test the specificity of different species.

    • Intra-Assay Precision (Precision within an assay)
      Three samples of known concentration were tested fifteen times on one plate to assess intra-assay precision.
      Inter-Assay Precision (Precision between assays)
      Three samples of known concentration were tested in separate assays to assess inter-assay precision. Assays were performed with at least three lots of components.

    • Determination of Minimum Detectable Dose (MDD)
      The MDD was determined using three independent lots of assay components. For each lot, 19 replicate measurements of the diluent (zero calibrator) were performed. The mean (AVERAGE) and standard deviation (STDEV) of the 19 replicates were calculated. The MDD for each lot was then calculated according to the following formula:
      MDD = 2 × STDEV + AVERAGE

    • HOOK Effect Threshold
      The upper limit of the HOOK effect was established at 100× the highest calibrator concentration (equivalent to a 2-log10 increase). No HOOK effect was observed below this threshold, confirming that samples within this concentration range yield reliable quantitative results without signal depression.

    • Leading Competitor comparison

    • Protocol Diagram

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