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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human & Monkey Hemoglobin Serum & Plasma OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3174
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    Hemoglobin

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu, Mk

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 1.9%
      Inter-assay: 2.7%

    • Sample type

      Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      1.353 ng/mL

    • Range

      8.78 ng/mL – 100 ng/mL

    • Recovery

      Human Serum: 108%
      Heparin Plasma: 81%
      EDTA Plasma: 93%

    • Assay time

      60 minutes

    • Species reactivity

      Hu; Mk

    • CROSS REACTIVITY

      Rt
背景介绍

  • Human hemoglobin is a complex metalloprotein found in red blood cells that is essential for transporting oxygen from the lungs to body tissues and facilitating the return transport of carbon dioxide. Structurally, it is a tetramer consisting of two alpha and two beta polypeptide chains in adults (HbA), each bound to a heme group containing a ferrous iron atom that can reversibly bind one oxygen molecule, allowing a single hemoglobin molecule to carry up to four oxygen molecules. Functionally, hemoglobin exhibits cooperative binding, where the binding of one oxygen molecule increases the affinity for the next, resulting in a sigmoidal oxygen dissociation curve modulated by factors such as pH (the Bohr effect), carbon dioxide, and 2,3-bisphosphoglycerate (2,3-BPG) to adapt oxygen release to tissue metabolic needs. Mutations in the globin genes can lead to disorders like sickle cell disease and thalassemias, making the understanding of hemoglobin’s structure and function fundamental to both physiology and clinical medicine.

  • ELISA

    • Linearity
      The concentrations of Human hemoglobin were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: Human serum was 10fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 535.69 ng/mL in human serum.
      #2 sample is undiluted samples are as follows: Human plasma-EDTA was 2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 256.82 ng/mL in Human plasma-EDTA.
      #3 sample is undiluted samples are as follows: Human plasma-EDTA-donor#1 was 2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 53.44 ng/mL in Human plasma-EDTA donor#1.
      #4 sample is undiluted samples are as follows: Human plasma-EDTA-donor#2 was 3fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 390.37 ng/mL in Human plasma-EDTA-donor#2.
      #5 sample is undiluted samples are as follows: Human plasma -Heparin was 15fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1810.71 ng/mL in Human plasma -Heparin.

    • Linearity
      The concentrations of Human hemoglobin were measured and interpolated from the target standard curves and corrected for sample dilution.
      #6 sample is undiluted samples are as follows: Female monkey serum was 10fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 465.74 ng/mL in female monkey serum.

    • Linearity
      The concentrations of Human hemoglobin were measured and interpolated from the target standard curves and corrected for sample dilution.
      #7 sample is undiluted samples are as follows: SD rat serum was 10fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 338.19 ng/mL in SD rat serum.

    • To assess the linearity of the assay, the sample was spiked with high concentrations of Human hemoglobin in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, the sample was spiked with high concentrations of Human hemoglobin in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Protocol Diagram

    • Standard curve
      Example of Human hemoglobin standard curve in Assay Diluent DC4.

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