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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Human CCL22/MDC OneStep ELISA Kit

价格 ￥3,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3172
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    CCL22

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Hu

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 2.4%
      Inter-assay: 3.5%

    • Sample type

      Cell culture supernatant; Serum; Plasma

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      2.162 pg/mL

    • Range

      6.25 pg/mL – 400 pg/mL

    • Recovery

      Cell culture supernatant: 104%
      Serum: 104%
      EDTA Plasma: 107%

    • Assay time

      60 minutes

    • Species reactivity

      Hu
背景介绍

  • Human CCL22 (C-C motif chemokine ligand 22), also known as macrophage-derived chemokine (MDC), is a small secretory protein composed of approximately 67-69 amino acids. Its gene is located on chromosome 16. It exerts its biological functions primarily by interacting with its receptor CCR4, thereby chemotactically recruiting various immune cells, including regulatory T cells (Tregs), Th2 cells, and natural killer (NK) cells, playing a crucial role in maintaining immune homeostasis and mediating inflammatory responses.

  • ELISA

    • Standard curve
      Example of Human CCL22/MDC standard curve in Assay Diluent D1.

    • Linearity
      The concentrations of Human CCL22 were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: human PBMC cells stimulated with 10ug/ml PHA for 5days (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 189.1 pg/mL in stimulated human PBMC supernatant.
      #2 sample is undiluted samples are as follows: human PBMC cells culture for 5days (50%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 162.96 pg/mL in unstimulated human PBMC supernatant.

    • #3 sample is undiluted samples are as follows: Human serum was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 1041.61 pg/mL in human serum.
      #4 sample is undiluted samples are as follows: Human plasma-EDTA was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 399.44 pg/mL in human plasma-EDTA.

    • Protocol Diagram

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