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产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

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Rat alpha 2-Macroglobulin OneStep ELISA Kit

价格 ￥3,000.00 （ 1-2周）
货号 S0C3171
规格
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产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    alpha 2-Macroglobulin

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Rt

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 2.3%；
      Inter-assay: 3.7%

    • Sample type

      Cell Culture Supernatant; Serum; EDTA Plasma, Heparin Plasma, Citrate Plasma, Tissue Extracts

    • Assay type

      Sandwich (quantitative)

    • Sensitivity

      34.6 pg/mL

    • Range

      78.1 pg/mL – 5000 pg/mL

    • Recovery

      Cell culture supernatant: 96%
      Serum: 94%
      EDTA Plasma: 101%
      Heparin Plasma: 107%
      Citrate Plasma: 96%
      Tissue Extracts: 98%

    • Assay time

      60 minutes

    • Species reactivity

      Rt
背景介绍

  • Rat alpha 2-Macroglobulin (A2M) is a large plasma protein that functions as a broad-spectrum protease inhibitor. It is primarily synthesized in the liver and circulates in the blood as a homotetrameric complex. Unlike smaller protease inhibitors, A2M inhibits proteases from all four catalytic classes by a unique entrapment mechanism. Upon cleavage of its bait region, the protein undergoes a major conformational change that physically encloses the target protease. This entrapped enzyme remains active toward small substrates but is blocked from accessing large protein substrates. Rat A2M also binds and facilitates the clearance of various growth factors, cytokines, and toxins through receptor-mediated endocytosis via the low-density lipoprotein receptor-related protein (LRP). Thus, it plays a critical role in modulating inflammatory responses, tissue remodeling, and host defense in rats.

  • ELISA

    • Standard curve
      Example of Rat alpha 2-Macroglobulin standard curve in Assay Diluent A1.

    • Linearity
      The concentrations of Rat alpha 2-Macroglobulin were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows: Rat serum was 20000-fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 20.281 μg/mL in Rat serum.

    • Linearity
      The concentrations of Rat alpha 2-Macroglobulin were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows: Rat EDTA plasma was 1000-fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 7.384 μg/mL in Rat EDTA plasma.
      Sample is undiluted samples are as follows: Rat Citrate plasma was 1000-fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 6.433 μg/mL in Rat Citrate plasma.
      Sample is undiluted samples are as follows: Rat Heparin Plasma was 1000-fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 7.209 μg/mL in Rat Heparin Plasma.

    • Linearity
      The concentrations of Rat alpha 2-Macroglobulin were measured and interpolated from the target standard curves and corrected for sample dilution.
      Sample is undiluted samples are as follows: Rat skeletal muscle tissue lysate was 500 μg/mL. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 44.865 ng/mL in Rat skeletal muscle tissue lysate.

    • To assess the linearity of the assay, two samples were spiked with high concentrations of Rat alpha 2-Macroglobulin in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • To assess the linearity of the assay, sample was spiked with high concentrations of Rat alpha 2-Macroglobulin in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

    • Specificity
      Test the specificity of different serum.

    • Serum titer
      Select different species of serum for serum titer experiments, with a dilution ratio of 10.

    • Linearity (Species crossing)
      Sample is undiluted samples are as follows: Mouse serum was 100-fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 608.8 ng/mL in Mouse serum.

    • Linearity (Species crossing)
      Sample is undiluted samples are as follows: CAVPO serum was 40-fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 93.9 ng/mL in CAVPO serum.

    • Linearity (Species crossing)
      Sample is undiluted samples are as follows: Human serum was 4-fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 16.3 ng/mL in Human serum.

    • Linearity (Species crossing)
      Sample is undiluted samples are as follows: Rabbit serum was 8-fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 94.0 ng/mL in Rabbit serum.

    • Leading Competitor comparison

    • Protocol Diagram

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