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抗原名称
C-peptide免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Hu, Mk纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8°C as supplied.
Precision
Intra-assay: 1.4%
Inter-assay: 2.7%Sample type
Cell culture supernatant; Serum; PlasmaAssay type
Sandwich (quantitative)Sensitivity
10.917 pg/mLRange
46.88 pg/mL – 3000 pg/mL (0.16 nmol/L – 1.00 nmol/L)Recovery
Cell culture supernatant: 91%
Human Serum: 119%
Citrate Human plasma: 97%Assay time
60 minutesSpecies reactivity
Hu; Mk
Human C-peptide is a 31-amino-acid peptide that connects the A and B chains of proinsulin in the human pancreas, released into the bloodstream in equimolar amounts to insulin and serving as a critical marker of endogenous insulin secretion; clinically, its measurement helps distinguish between type 1 and type 2 diabetes, evaluate residual beta-cell function, diagnose insulinomas, and assess causes of hypoglycemia, since synthetic insulin preparations do not contain C-peptide. Monkey C-peptide is highly similar to human C-peptide in both structure and function, reflecting the close phylogenetic relationship between humans and non-human primates—Old World monkeys (e.g., macaques) typically have a C-peptide sequence differing from humans by only a few amino acids while maintaining comparable bioinertness and stoichiometric release with insulin. In preclinical research and toxicology studies, monkey C-peptide is measured as a translational biomarker to evaluate pancreatic beta-cell health, insulin secretion dynamics, and the diabetogenic potential of novel drugs. Thus, both human and monkey C-peptides are valuable biomarkers for insulin production, and their structural homology supports the use of monkeys as relevant models for human metabolic and diabetic research; however, species-specific assays are often required for accurate quantification due to minor sequence variations.
ELISA
Standard curve
Example of Human C-peptide standard curve in Assay Diluent DD5.Standard curve
Example of Human C-peptide standard curve in Assay Diluent DD5.Linearity
The concentrations of Human C-peptide were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Human serum donor #1 was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3255.81 pg/mL in human serum donor #1.
#2 sample is undiluted samples are as follows: Human serum donor #2 was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3113.40 pg/mL in human serum donor #2.
#3 sample is undiluted samples are as follows: Human plasma-EDTA was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3817.99 pg/mL in human plasma-EDTA.#4 sample is undiluted samples are as follows: Female monkey serum was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 160.46 pg/mL in female monkey serum.
#5 sample is undiluted samples are as follows: Monkey plasma-EDTA was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 858.96 pg/mL in monkey plasma-EDTA.Protocol Diagram
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