热搜词：
注册
登录
CN
  • CN
  • EN

产品中心 ELISA试剂盒 OneStep ELISA 试剂盒

所有图片（1/4）

Monkey IgE Competitive ELISA Kit

价格 ￥4,000.00 （ 供应商现货 ： 3-5个工作日）
货号 S0C3166
规格
数量
收藏 分享
产品介绍

产品介绍 评论(0)

产品规格

  • 抗原名称

    IgE

  • 免疫原

    Recombinant Protein

  • 抗体类型

    Recombinant mAb

  • 反应种属 ?

    Mk

  • 纯化方式

    Protein A

  • 储存条件

    12 months from date of receipt / reconstitution, 2 to 8°C as supplied.

试剂盒参数

    • Precision

      Intra-assay: 3.4%
      Inter-assay: 7.1%

    • Sample type

      Cell culture supernatant; Serum; Plasma

    • Assay type

      Competitive

    • Sensitivity

      <312.5 pg/mL

    • Range

      312.5 ng/mL – 20,000 ng/mL

    • Recovery

      Cell culture supernatant: 108%
      Monkey Serum: 118%
      Heparin Monkey Plasma: 99%
      EDTA Monkey Plasma: 98%
      Citrate Monkey Plasma: 95%

    • Assay time

      60 minutes

    • Species reactivity

      Mk; Hu
背景介绍

  • Immunoglobulin E (IgE) in monkeys is a class of antibodies that plays a central role in allergic responses and defense against parasitic infections, closely resembling its human counterpart in both structure and function. Like human IgE, monkey IgE binds to high-affinity receptors (FcεRI) on mast cells and basophils; when cross-linked by allergens or parasite antigens, it triggers the release of inflammatory mediators such as histamine and leukotrienes, leading to typical allergic symptoms like itching, swelling, and bronchoconstriction. Due to the phylogenetic and immunological proximity between monkeys (especially macaques) and humans, monkey IgE is a valuable tool in biomedical research, enabling the study of human allergic diseases, asthma, anaphylaxis, and the testing of anti-allergic drugs or vaccine-induced immune responses, while also helping to manage allergic conditions in laboratory primates.

  • ELISA

    • Standard curve
      Example of Monkey IgE standard curve in Assay Diluent EB4.

    • Linearity
      The concentrations of Monkey IgE were measured and interpolated from the target standard curves and corrected for sample dilution.
      #1 sample is undiluted samples are as follows: Male monkey serum was 2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 2451.12 ng/mL in male monkey serum.
      #2 sample is undiluted samples are as follows: Female monkey serum was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 3027.71 ng/mL in female monkey serum.

    • #3 sample is undiluted samples are as follows: Human serum was 2fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 596.94 ng/mL in human serum.

    • Protocol Diagram

评论(0)