产品中心 ELISA试剂盒 OneStep ELISA 试剂盒
分享产品介绍 评论(0)
抗原名称
IL-4R免疫原
Recombinant Protein抗体类型
Recombinant mAb反应种属 ?
Ms纯化方式
Protein A储存条件
12 months from date of receipt / reconstitution, 2 to 8°C as supplied.
Precision
Intra-assay: 1.3%
Inter-assay: 6.2%Sample type
Cell culture supernatant; Serum; Plasma; Tissue ExtractsAssay type
Sandwich (quantitative)Sensitivity
9.425 pg/mLRange
31.25 pg/mL – 2000 pg/mLRecovery
Cell culture supernatant: 112%
Mouse Serum: 86%
Heparin Plasma: 96%
EDTA Plasma: 104%
Citrate Plasma: 106%
Tissue Extracts: 110%Assay time
60 minutesSpecies reactivity
Ms
Mouse Interleukin-4 Receptor (IL-4R) is a critical transmembrane protein that orchestrates immune responses by binding its primary ligands IL-4 and IL-13, functioning through two distinct receptor complexes—the type I receptor heterodimerizing with the common gamma chain and the type II receptor pairing with IL-13Rα1—to activate downstream Jak-STAT6 signaling pathways that drive Th2 cell differentiation, B-cell class switching to IgE, and the expression of genes essential for allergic inflammation and helminth defense, making this receptor system indispensable for understanding adaptive immunity and a pivotal target for therapeutic intervention in mouse models of allergic diseases, autoimmune disorders, and cancer immunotherapy research.
ELISA
Standard curve
Example of Mouse IL-2 standard curve in Assay Diluent SR4.Linearity
The concentrations of Mouse IL-4R were measured and interpolated from the target standard curves and corrected for sample dilution.
#1 sample is undiluted samples are as follows: Mouse spleen cells stimulated with 100 ng/ml IFN-γ and 1 μg/mL LPS for 3days (100%). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 59.46 pg/mL in stimulated mouse spleen cell supernatant.#2 sample is undiluted samples are as follows: Balb/c mouse serum was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 14.24 ng/mL in Balb/c mouse serum.
#3 sample is undiluted samples are as follows: Balb/c mouse citrate plasma was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 12.76 ng/mL in Balb/c mouse citrate plasma.
#4 sample is undiluted samples are as follows: CD-1 mouse serum was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 9.79 ng/mL in CD-1 mouse serum.
#5 sample is undiluted samples are as follows: CD-1 mouse citrate plasma was 4fold dilution. The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 14.52 ng/mL in CD-1 mouse citrate plasma.#6 sample is undiluted samples are as follows: C57BL/6 mouse spleen extracts was 4fold dilution (1 mg/mL). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 45.73 pg/mL in C57BL/6 mouse spleen extracts (1 mg/mL).
#7 sample is undiluted samples are as follows: Balb/c mouse spleen extracts was 4fold dilution (1 mg/mL). The interpolated dilution factor corrected values are plotted. The mean target concentration was determined to be 180.85 pg/mL in Balb/c mouse spleen extracts (1 mg/mL).To assess the linearity of the assay, the sample was spiked with high concentrations of Mouse IL-4R in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
To assess the linearity of the assay, two samples were spiked with high concentrations of Mouse IL-4R in various matrices and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Specificity
Test the specificity of different serum.Protocol Diagram
评论(0)